Structure of PDB 7qbc Chain B

Receptor sequence

>7qbcB (length=298) Species: 4932 (Saccharomyces cerevisiae)

APSLSNLFYDPTYNPGQSTINYTSIYGNGSTITFDELQGLVNSTVTQAIM
FGVRCGAAALTLIVMWMTSRSRKTPIFIINQVSLFLIILHSALYFKYLLS
NYSSVTYALTGFPQFISRGDVHVYGATNIIQVLLVASIETSLVFQIKVIF
TGDNFKRIGLMLTSISFTLGIATVTMYFVSAVKGMIVTYNDVSATQDKYF
NASTILLASSINFMSFVLVVKLILAIRSRRFLGLKQFDSFHILLIMSCQS
LLVPSIIFILAYSLKPNQGTDVLTTVATLLAVLSLPLSSMWATAANNA

3D structure

• PDB: 7qbc Activation mechanism of the class D fungal GPCR dimer Ste2.
• Chain: B
• Resolution: 3.53 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	Q51 H94 Y98 Y101 Y128 T131 N132 Q135 Y181 S184 A185 K202 F204 N205 I263 Y266 S267 K269 P270 N271 D275	Q47 H90 Y94 Y97 Y124 T127 N128 Q131 Y177 S180 A181 K198 F200 N201 I259 Y262 S263 K265 P266 N267 D271
BS02	Y01	B	L64 M71	L60 M67
BS03	Y01	B	L64 F99 L103	L60 F95 L99
BS04	Y01	B	T72 R74 C252 L291	T68 R70 C248 L287
BS05	Y01	B	F89 L93 V127	F85 L89 V123

Gene Ontology

Molecular Function

• GO:0004930 G protein-coupled receptor activity
• GO:0004932 mating-type factor pheromone receptor activity
• GO:0004934 mating-type alpha-factor pheromone receptor activity
• GO:0005515 protein binding

Biological Process

• GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion
• GO:0000755 cytogamy
• GO:0007186 G protein-coupled receptor signaling pathway
• GO:0019236 response to pheromone

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0038038 G protein-coupled receptor homodimeric complex

External links

• PDB: RCSB:7qbc, PDBe:7qbc, PDBj:7qbc
• PDBsum: 7qbc
• PubMed: 35296853
• UniProt: D6VTK4|STE2_YEAST Pheromone alpha factor receptor (Gene Name=STE2)