Structure of PDB 7pt2 Chain B

Receptor sequence

>7pt2B (length=572) Species: 1120948 (Actinomycetospora chiangmaiensis DSM 45062)

RQSVPVASLVAEFLQEHGVDRVFGLQGGHIQPIWDQLARRGVRIVDVRDE
GSAVHMAHAHTELTGQTAVAMVTAGPGVTNTVTAVANASVSRIPLLVIGG
CPPIPQSNMGPLQDIPHTAILEPITRLARTLRSADQVLREFDEAWARASG
DRGEPGPVYLEIPTDVLRRDVPPALQMREHLRAKPKRRPQPHPDDVAAVA
DLIRAAEKPAIISGRGARTTDGTDLVRLLDASGAAYLDTQESRGLVPDSH
PAAVGSARSAVMRDTDLLITVGRQLDYQLGMGSPAVFPHAKVVRIADTAS
ELIDNRRGEVEILAEPGAALAAIADALKDHTPDTSWRDELKAKHRKRAED
YRQALHSTENGADGHIHPNRIFGALDALDGDVLDLGETIMIADGGDLLSF
GRLGITKARRYLDAGAFGCLGVATPFAIGAALAYPDRPVVAVTGDGAFGI
TATEIDTAVRHDAKIVVIVSNNRAWNIQRYDQAENYGLVVGTDLADSDYA
GVARAFGAHGERVTDPAELEGAIRRALANAPALVDVVTTQDAASPDSGKG
LGFVPDYQALTPWNDAEVARRQ

3D structure

• PDB: 7pt2 Mechanistic details of the actinobacterial lyase-catalyzed degradation reaction of 2-hydroxyisobutyryl-CoA.
• Chain: B
• Resolution: 1.762 Å

Enzymatic activity

• Enzyme Commision number: 4.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TPW	B	E65 T88	E50 T73
BS02	3KK	B	L127 Q128	L112 Q113
BS03	TPW	B	G410 D411 L412 L435 G459 G461 A462 N487 A489 W490 N491 I492 Q493	G395 D396 L397 L420 G444 G446 A447 N472 A474 W475 N476 I477 Q478
BS04	3KK	B	G270 S271 R273 S274 Q293 R362 Y366 R417 L418 G433 Q493 D561 L566	G255 S256 R258 S259 Q278 R347 Y351 R402 L403 G418 Q478 D546 L551
BS05	MG	B	D460 N487 A489	D445 N472 A474

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0003984 acetolactate synthase activity
• GO:0016829 lyase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0046872 metal ion binding
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0009097 isoleucine biosynthetic process
• GO:0009099 L-valine biosynthetic process

Cellular Component

• GO:0005948 acetolactate synthase complex

External links

• PDB: RCSB:7pt2, PDBe:7pt2, PDBj:7pt2
• PDBsum: 7pt2
• PubMed: 34952003
• UniProt: P0DUV9|HACL_ACTC0 2-hydroxyacyl-CoA lyase