Structure of PDB 7p8n Chain B

Receptor sequence

>7p8nB (length=613) Species: 243274 (Thermotoga maritima MSB8) [Search protein sequence]

MFKNAKEFVQYANKLKTLREKKLNGVSIYVCVGTGCTAKGALKVYSAFEE
ELKKRNLLKVTLNRTGCCGRCSSGPLVKIMPYRFFYSNVAPEDVPEIVDR
TVLKGEPIERLFLTDPLTGEKVPRIEDTTLFKNQDFYIMEAIGESECDSI
EDYIARSGYESLVKALTSMTPEEIIETVKASGLRGRGGGGFPTGLKWEFT
RKAQGDIKFVVCNGDEGDPGAFMNRTLLERDPHLVLEGMIIAGYAVGAQK
GYAYIRAEYPFAVKMFKKAIEDARKLGLLGENILGTGFSFDLEVKEGAGA
FVCGEETALLASIEGKRGMPRPKPPFPAQSGLWGKPTLINNVETYANIPR
ILRDGVENYRKRGTENSPGTKMFSVAGPLKATGIIEVEFGTTLRDIIYNI
CGGFVEGEEFKAVQIGGPSGACLSEDFIDMPLDYDTLKKADAMVGSGGIV
VITKKTCMVEVARFFLDFTKRESCGKCVPCREGTMQAYNILEKFTHGKAT
YEDLKTLEHLSKTIKTASLCGLGKTAPNPILSTLKLFREEYIAHIEGECP
SGMCTAFKKYVINPDICKGCGLCARSCPQNAITGERGKPYTIDQEKCVKC
GLCASKCPFKAIE

3D structure

PDB

7p8n Structural insight on the mechanism of an electron-bifurcating [FeFe] hydrogenase.

Chain

Resolution

2.8 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

1.12.1.4: hydrogenase (NAD(+), ferredoxin).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	SF4	B	Y571 C588 I603 C608 K610 C611 G612 C614	Y560 C577 I592 C597 K599 C600 G601 C603
BS02	SF4	B	C578 C581 G582 C584 Y601 C618	C567 C570 G571 C573 Y590 C607
BS03	SF4	B	P331 S484 C485 G486 K487 C488 C491 L530 C531 L533 G534	P320 S473 C474 G475 K476 C477 C480 L519 C520 L522 G523
BS04	FMN	B	G196 R197 G198 N224 D226 E227 G228 F312 G315 E316 E317 N351 N352 G532 L533	G185 R186 G187 N213 D215 E216 G217 F301 G304 E305 E306 N340 N341 G521 L522
BS05	FES	B	C31 G33 T34 C36 C78 C79 G80 R81 C82	C31 G33 T34 C36 C67 C68 G69 R70 C71
BS06	ZN	B	C468 H555 C560 C565	C457 H544 C549 C554

Gene Ontology

	Molecular Function
GO:0008137	NADH dehydrogenase (ubiquinone) activity
GO:0010181	FMN binding
GO:0016491	oxidoreductase activity
GO:0046872	metal ion binding
GO:0051539	4 iron, 4 sulfur cluster binding

	Biological Process
GO:1902600	proton transmembrane transport

	Cellular Component
GO:0005737	cytoplasm

View graph for
Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:7p8n, PDBe:7p8n, PDBj:7p8n
PDBsum	7p8n
PubMed	36018003
UniProt	O52682\|HYDB_THEMA Bifurcating [FeFe] hydrogenase beta subunit (Gene Name=hydB)

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