Structure of PDB 7oo3 Chain B

Receptor sequence

>7oo3B (length=1131) Species: 9823 (Sus scrofa)

DEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPP
IDLQAPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLY
VDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELN
ECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCL
ENSSRPTSTIWVSMLARGAIGQRIVATLPYIKQEVPIIIVFRALGFVSDR
DILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTK
EKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRREL
DDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLE
LAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHL
RRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYI
SVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQ
LMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQK
LLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDL
QEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQA
MGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAI
ASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKP
TRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPRYTKRDCS
TFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKG
TCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANK
GEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFI
GPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDC
QIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNK
TQISLVRMPYACKLLFQELMSMSIAPRMMSV

3D structure

• PDB: 7oo3 Structural basis of human transcription-DNA repair coupling.
• Chain: B
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	R222 R385 D386 I489 L495 P498	R197 R353 D354 I457 L463 P466
BS02	dna	B	A449 T450 V469 T746 K821 G1077 R1078 L1084 R1085 M1089	A417 T418 V437 T714 K789 G1034 R1035 L1041 R1042 M1046
BS03	rna	B	A464 G465 Q468 Q731 K934 K942 H1053 R1080	A432 G433 Q436 Q699 K891 K899 H1010 R1037
BS04	ZN	B	C1119 C1122 C1137 C1140	C1076 C1079 C1094 C1097

Gene Ontology

Molecular Function

• GO:0001055 RNA polymerase II activity
• GO:0003677 DNA binding
• GO:0003682 chromatin binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0006366 transcription by RNA polymerase II

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0000781 chromosome, telomeric region
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005665 RNA polymerase II, core complex

External links

• PDB: RCSB:7oo3, PDBe:7oo3, PDBj:7oo3
• PDBsum: 7oo3
• PubMed: 34526721
• UniProt: I3LGP4