Structure of PDB 7nsn Chain B

Receptor sequence

>7nsnB (length=1224) Species: 220687 (Neobacillus novalis)

SKTDFFSSFEKSDLQLTWTNTVETDANGKKMSSGIDGNVAPPPGDMKSLI
GKGPTSSYTAKTNVGWTGLGALNYSGTHLSDGRAYSYNKLYDVDILVTPA
TELSYFIAPEFTDKNHNDYSSTYVSVDLAFSDGTYLHDLKAVDQYGVGLN
PKDQGDSKYLYVNQWNTIKSTIGSVAAGKTIKRILVAYDNPKGPGAFRGS
IDDIKIDGKPVQKAFGSPIDYVNILRGTQSNGSFSRGNNFPAVAIPHGFN
FWTPTTNAGSSWIYQYHESNSVNNLPQIQAFSVSHEPSPWMGDRQTFQVM
PSASTAATPNANRDSRALEFNHANEIAQPHYYSVKFENGIRTEMTPTDHA
AMFKFTFTGATSNLIFDNVNNNGGLTIDAKSGEITGYSDVKSGLSTGATR
LFVYAAFDKPVIKSGKLTGESRNNVTGYVRFDTSKDEDKVVTMKIATSLI
SVEQAKKNLEQEIGLNDTFEGLKEKAKTEWNKKLGIIEVEGASEDQLVTL
YSNLYRLFLYPNSAFENVGTTTDPVYKYASPYSAATGQDTATTTGAKIVD
GKTYVNNGFWDTYRTAWPAYSLLTPTFAGELIDGFVQQYRDGGWIARWSS
PGFANLMPGTSSDVAFADAYLKGVTNFDVQSFYQSAIRNAEAVSPNAGTG
RKGLTTSIFDGYTNTSTGEGLAWAMDGYINDFGIANLAKALKEKGDKSDP
YYANYAADYQYFLNRAQNYVHMFNPSIEFFNGRTANGAWRSTPDNFNPAV
WGSDYTETNGWNMAFHVPQDGQGLANLYGGKEGLATKLDQFFSTSETGLF
PGSYGGTIHEMREARDVRMGMYGHSNQPSHHIAYMYDYAGQPWKTQEKVR
EALNRLYIGSAIGQGYSGDEDNGEMSAWYILSAMGFYPLKMGTPEYAIGA
PLFKKATIHLENGKSIVINAPNNSKENKYVQSMKVNGKAYAKTSILHADI
ANGAVIDFEMGSKPSKWGSGDQDILQSITPGSTDGTSLSPLPLRDVTDRL
IAAEKGAVTVSDEGNGQLLFDNTSNTQLSMKSKTPSIVYQFKEGKQNVKM
YTLTSSKASQNEDPKSWVLKGSNDGKSWSVLDQRKNETFQWRQYTRAFTI
QHPGKYSQYKLEITENAGAEVTTLAELELLGYDDVTNSYQAVYELMEQFK
QSKDLTGPMAVQLNNSLTTSLDHFKKDHKDQAIKHLEDFLKHLNNKGLQD
RISSKAKGVLSADANQLIVLLARD

3D structure

• PDB: 7nsn Structural and functional characterization of multi-domain GH92 alpha-1,2-mannosidase from Neobacillus novalis
• Chain: B
• Resolution: 2.29 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	B	N1013 Q1014 D1058	N826 Q827 D871
BS02	CA	B	S48 E50 T254 G257 D389	S8 E10 T67 G70 D202
BS03	CA	B	D1182 D1185 N1209	D995 D998 N1022
BS04	CA	B	L1205 D1208 T1210 T1213 A1312 E1313	L1018 D1021 T1023 T1026 A1125 E1126
BS05	MVL	B	S475 W477 W747 D748 W785 M794 E944 D1058	S288 W290 W560 D561 W598 M607 E757 D871

Gene Ontology

Molecular Function

• GO:0030246 carbohydrate binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:7nsn, PDBe:7nsn, PDBj:7nsn
• PDBsum: 7nsn
• PubMed: 37071393
• UniProt: A0A7R9KAR8