Structure of PDB 7mid Chain B

Receptor sequence
>7midB (length=340) Species: 243231 (Geobacter sulfurreducens PCA) [Search protein sequence]
GRGLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARLGETSQVALFGNATL
TTAALHECLRREIPVTWLSYGGWFMGHTVSTGHRNVETRTYQYQRSFDPE
TCLNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPPGLLMSLQDDMRHAM
RAPSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMGFDFTTRNRRPPKDP
VNALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPRFGRPALALDMMEPF
RPLIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSARKRFIAGFERRMEQ
EVTHPIFKYTISYRRLLEVQARLLTRYLSGEIPAYPNFVT
3D structure
PDB7mid Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
ChainB
Resolution3.56 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B P229 K230 Y232 E243 R246 N265 P11 K12 Y14 E25 R28 N47
BS02 dna B S287 R505 I509 S69 R287 I291
BS03 MN B E380 E466 E162 E248
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0004527 exonuclease activity
GO:0046872 metal ion binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:7mid, PDBe:7mid, PDBj:7mid
PDBsum7mid
PubMed34588691
UniProtQ74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)

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