Structure of PDB 7mib Chain B

Receptor sequence
>7mibB (length=338) Species: 243231 (Geobacter sulfurreducens PCA) [Search protein sequence]
GLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARLGETSQVALFGNATLTT
AALHECLRREIPVTWLSYGGWFMGHTVSTGHRNVETRTYQYQRSFDPETC
LNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPPGLLMSLQDDMRHAMRA
PSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMGFDFTTRNRRPPKDPVN
ALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPRFGRPALALDMMEPFRP
LIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSARKRFIAGFERRMEQEV
THPIFKYTISYRRLLEVQARLLTRYLSGEIPAYPNFVT
3D structure
PDB7mib Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
ChainB
Resolution5.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B K230 Y232 R246 K10 Y12 R26
BS02 dna B Y288 R412 R413 P415 R454 R457 N499 Y68 R192 R193 P195 R234 R237 N279
BS03 dna B E374 G378 A382 R386 E154 G158 A162 R166
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0004527 exonuclease activity
GO:0046872 metal ion binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:7mib, PDBe:7mib, PDBj:7mib
PDBsum7mib
PubMed34588691
UniProtQ74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)

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