Structure of PDB 7mi9 Chain B

Receptor sequence

>7mi9B (length=338) Species: 243231 (Geobacter sulfurreducens PCA)

GLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARLGETSQVALFGNATLTT
AALHECLRREIPVTWLSYGGWFMGHTVSTGHRNVETRTYQYQRSFDPETC
LNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPPGLLMSLQDDMRHAMRA
PSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMGFDFTTRNRRPPKDPVN
ALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPRFGRPALALDMMEPFRP
LIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSARKRFIAGFERRMEQEV
THPIFKYTISYRRLLEVQARLLTRYLSGEIPAYPNFVT

3D structure

• PDB: 7mi9 Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
• Chain: B
• Resolution: 3.89 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-
  3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	P229 K230 Y232 E244 E245 R246 N265 T267 E374 V375 G378 I379 F455	P9 K10 Y12 E24 E25 R26 N45 T47 E154 V155 G158 I159 F235
BS02	dna	B	S287 Y288 G289 W291 L377 R410 N411 R412 R413 P414 S424 F425 Y427 A428 R432 H451 Q452 R454 R457 L462 R469 N499	S67 Y68 G69 W71 L157 R190 N191 R192 R193 P194 S204 F205 Y207 A208 R212 H231 Q232 R234 R237 L242 R249 N279
BS03	dna	B	L377 E380 G381 A384 G385 F388 Q389 F407 T408 R410 R412	L157 E160 G161 A164 G165 F168 Q169 F187 T188 R190 R192

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004527 exonuclease activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:7mi9, PDBe:7mi9, PDBj:7mi9
• PDBsum: 7mi9
• PubMed: 34588691
• UniProt: Q74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)