Structure of PDB 7mei Chain B

Receptor sequence
>7meiB (length=1166) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
DESAPITAEDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQDIICEDSTL
ILEQLAQHTTESDNISRKYEISFGKIYVTKPMVNESDGVTHALYPQEARL
RNLTYSSGLFVDVKTYEAIDVPGRELKYEDSESGKVFIGRLPIMLRSKNC
YLSEATESDLYKLKECPFDMGGYFIINGSEKVLIAQERSAGNIVQVFKKA
APSPISHVAEIRSALEKGSRFISTLQVKLYGRSSARTIKATLPYIKQDIP
IVIIFRALGIIPDGEILEHICYDVNDWQMLEMLKPCVEDGFVIQDRETAL
DFIGRRGTALGIKKEKRIQYAKDILQKEFLPHITQLEGFESRKAFFLGYM
INRLLLCALDRKDQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRY
MQRTVEEAHDFNMKLAINAKTITSGLKYALATGNWGEQKKAMSSRAGVSQ
VLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQ
ACGLVKNLSLMSCISVGTDPMPIITFLSEWGMEPLEDYVPHQSPDATRVF
VNGVWHGVHRNPARLMETLRTLRRKGDINPEVSMIRDIREKELKIFTDAG
RVYRPLFIVEDDESLGHKELKVRKGHIAKLMATEYQDEYTWSSLLNEGLV
EYIDAEEEESILIAMQPEDLEPAAKRIRATTFTHCEIHPSMILGVAASII
PFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYYPQKPLGTT
RAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFF
RSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVS
GEDVIIGKTTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQVLVTT
NQDGLKFVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGI
VPDLIINPHAIPSRMTVAHLIECLLSKVAALSGNEGDASPFTDITVEGIS
KLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPTYYQRLRHMVDDKIHAR
ARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAASFLKERLMEASD
AFRVHICGICGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLFQE
LMAMNIPRLYTDRSRD
3D structure
PDB7mei Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II.
ChainB
Resolution3.54 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B R241 S248 I251 Y275 K426 I502 G867 M868 S869 R212 S219 I222 Y244 K395 I471 G811 M812 S813
BS02 dna B G1121 R1122 S1123 L1128 R1129 G1065 R1066 S1067 L1072 R1073
BS03 rna B Q481 Q776 K979 K987 R1020 Q450 Q720 K923 K931 R964
BS04 ZN B C1163 C1166 C1185 C1107 C1110 C1129
Gene Ontology
Molecular Function
GO:0001055 RNA polymerase II activity
GO:0003677 DNA binding
GO:0003729 mRNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0003968 RNA-dependent RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0001172 RNA-templated transcription
GO:0006351 DNA-templated transcription
GO:0006366 transcription by RNA polymerase II
GO:0006367 transcription initiation at RNA polymerase II promoter
GO:0006368 transcription elongation by RNA polymerase II
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005665 RNA polymerase II, core complex
GO:0005739 mitochondrion
GO:0010494 cytoplasmic stress granule

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7mei, PDBe:7mei, PDBj:7mei
PDBsum7mei
PubMed35051353
UniProtP08518|RPB2_YEAST DNA-directed RNA polymerase II subunit RPB2 (Gene Name=RPB2)

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