Structure of PDB 7lo5 Chain B

Receptor sequence

>7lo5B (length=1013) Species: 980427 (Deinococcus wulumuqiensis) [Search protein sequence]

SLQLVKKFQKRLEDIVAYGGTRNESSVRAAFQQLLSDWAEGSGLRLITEV
TQKAVAGNNVRPDGTLKDSLQQSRGYWESKDEADTLDDEIQKKLAKGYPR
DNIIFEDSRLAVLMQNGEEVQRVDMGDAGALAGLLKLFFEFEPPQVLEFR
KAVDHFKDEMPHLLKILREAADAAEQKADYRGERDHFVEIAKEAINPDFS
PRDAREMLIQHILTGDLFTSVFDNAQYHEDNNIAQQLQQLAATFYKGPVK
RDIAERTKRYYGAIQAAAAQIADHHEKQRFLKALYENFYRAYNPAGAERL
GIFYTPGEIVRFMIEATDTLLEKHFQKELADKGVEILDPATGTGTFITEL
IDFLPKAKLEQKYREELHCNELALLPYYIANLNIEATYAQKMGRYEEFRN
IVLVDTLDNTGFGLFGSVTAENLERAKRQNARPVRVIIGNPPYRANQANE
NDNNKNREYKEIDRRIKATYVAASTAQKTKLYDMYSRFLRWATDRLKEDG
IVAFVSNSSFIDSRTFDGFRKEVVKDFDHIYILDMKGNANTSGERRKREG
GNVFNDQIKVGVAVYFLVRSDTKIWYHAVPDFWRAREKLEWLKTTKFEDI
EFDHIRPDAKHNWLGQVDEENDWNEFLPVADKDTKQAKGLGQERAIFKLY
SLGVVTNRDEWVYSRAEDELADKVRYFIGRYNEIIKLPLGDLMSRNWEGD
IKMTRATIADAQSRKSYSLEKNSIVPSLYRPFDVLKMYFSKNLNEMQYQM
PSIFPKGVGENVVIALSGSPAAKPFQVLATDILPSLDLLEKTQCLPFYRY
TMNGERLNNITDYALKAFQTHYADTSISREDIFHYVYAVLHHPAYREKYA
LNLRQEFPRIPFYPEFGRWAAWGRELMALHIGFESVAPYPLKRTDEPPKN
DTPEALALAKKARLKVQRDAAKQPTGAVELDGLTTLAGIPAAAWAYKLGN
RSALEWVLERHKETTPKDATIREKFNTYRFADHKERVIDLLARVTTVSVE
TVRIVGEMPAETM

3D structure

PDB

7lo5 Coordination of phage genome degradation versus host genome protection by a bifunctional restriction-modification enzyme visualized by CryoEM.

Chain

Resolution

2.86 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	N24 E25 S26 R62 K97 Y99	N23 E24 S25 R61 K96 Y98
BS02	dna	B	F304 P449 Y451 R452 A484 Q485 K486 K488 N515 S517 S521 R522 T523 F562 Q565 I566 K567 V568 L668 M762 Y764 Q765 P786 K807	F303 P441 Y443 R444 A476 Q477 K478 K480 N507 S509 S513 R514 T515 F554 Q557 I558 K559 V560 L652 M746 Y748 Q749 P770 K791
BS03	dna	B	N454 Q455 N457 E458 K463 K475 Q485 K486 T487 Y490 A547 N548 T549 R554 R600 V671 N673 D675 K718 R721 R746 D803	N446 Q447 N449 E450 K455 K467 Q477 K478 T479 Y482 A539 N540 T541 R546 R584 V655 N657 D659 K702 R705 R730 D787
BS04	SAM	B	Y286 L301 G302 F304 T306 A341 T342 T344 T346 F347 E372 L373 D406 N448 P450 M492 F496	Y285 L300 G301 F303 T305 A340 T341 T343 T345 F346 E371 L372 D405 N440 P442 M484 F488
BS05	CA	B	E25 D64 E79 S80	E24 D63 E78 S79

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0004519	endonuclease activity
GO:0008168	methyltransferase activity
GO:0008170	N-methyltransferase activity
GO:0009007	site-specific DNA-methyltransferase (adenine-specific) activity

	Biological Process
GO:0009307	DNA restriction-modification system
GO:0032259	methylation

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:7lo5, PDBe:7lo5, PDBj:7lo5
PDBsum	7lo5
PubMed	33826880
UniProt	A0A345IJ72

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