Structure of PDB 7lhz Chain B

Receptor sequence

>7lhzB (length=631) Species: 507522 (Klebsiella pneumoniae 342)

PGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNT
WEVSSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLH
IATLLCALFVRHFRTLVKEGHVYVALPPLYQRFKGLGEMNPMQLRETTLD
PNTRRLVQLVISDEDEQQTTAIMDMLLAKKRSEDRRNWLQEKGSDMAERL
ALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRRIVYAMSELGLNASA
KFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDGQGNWGA
PDDPKSFAAMRYTESRLSKYAELLLSELGQGTVDWVPNFDGTLQEPKMLP
ARLPNILLNGTTGIAVGMATDIPPHNLREVAKAAITLIEQPKTTLDELLD
IVQGPDFPTEAEIITSRAEIRKIYQNGRGSVRMRAVLPHQVSGAKVLEQI
AAQMRNEQVMNHLFATTDLEKSYRINLNMIGLDGRPAVKNLLEILSEWLV
FRRDTVRRRLNHRLEKVLKRLHILEGLLVAFLNIDEVIEIIRTEDEPKPA
LMSRFGISETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQAILA
SERKMNNLLKKELQADADAFGDDRRSPLHER

3D structure

• PDB: 7lhz Discovery and Optimization of DNA Gyrase and Topoisomerase IV Inhibitors with Potent Activity against Fluoroquinolone-Resistant Gram-Positive Bacteria.
• Chain: B
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	E419 G443 K444 D495 R1029 V1041 Q1042 H1075 H1077 G1078 A1085 T1170 I1172	E21 G45 K46 D97 R221 V233 Q234 H267 H269 G270 A277 T362 I364
BS02	dna	B	L446 N447 S459 H498 L502 R616 Y1018 A1117 I1172 A1173 G1175 M1176 S1324	L48 N49 S61 H100 L104 R185 Y210 A309 I364 A365 G367 M368 S472
BS03	MG	B	D491 D493	D93 D95
BS04	Y21	B	K442 G443 E461	K44 G45 E63

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:7lhz, PDBe:7lhz, PDBj:7lhz
• PDBsum: 7lhz
• PubMed: 33929852
• UniProt: B5XU53;
  B5XU60