Structure of PDB 7kf3 Chain B

Receptor sequence

>7kf3B (length=362) Species: 359391 (Brucella abortus 2308)

MKKVALITGITGQDGAYLAELLLQKGYAVHGIKRRTSQFNTARIDHLYHD
PHEQGVDLTLHHGDLTDTSSLVRIMQLVRPDEVYNLGAQSHVAVSFEEPE
YTANSDALGALRLLEAIRILGMEKQTRYYQASTSELYGLVQEIPQKETTP
FYPRSPYAAAKLYAYWITVNYREAYGIYACNGILFNHESPLRGETFVTRK
ITRALARIKIGNQNRLYLGNLDSLRDWGHARDYVEMQWLMLQQDQPEDFV
IATGKQYSVREFVTLAAKDIGIEMRWEGSGEAEKGYDAKTGACIVEVDPR
YFRPAEVETLLGDATKAHQKLGWKPKISFETLVSEMIAEDLAAAARENLL
REHGHQFFAPKE

3D structure

• PDB: 7kf3 Crystal structure of GDP-mannose 4,6-dehydratase from Brucella abortus (strain 2308) in complex with Guanosine-diphosphate-rhamnose
• Chain: B
• Resolution: 2.35 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T133 S134 E135 Y157 K161
• Catalytic site (residue number reindexed from 1): T133 S134 E135 Y157 K161
• Enzyme Commision number: 4.2.1.47: GDP-mannose 4,6-dehydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAP	B	T36 S37	T36 S37
BS02	GDR	B	S90 V92 T133 E135 Y157 N186 R192 F196 V197 K200 L218 G219 N220 S223 R225 Y301 R303 E306	S90 V92 T133 E135 Y157 N186 R192 F196 V197 K200 L218 G219 N220 S223 R225 Y301 R303 E306
BS03	NAP	B	G9 T11 G12 Q13 D14 R34 N40 D64 L65 L86 G87 A88 S90 Y101 A131 T133 Y157 K161 N186 H187 R192	G9 T11 G12 Q13 D14 R34 N40 D64 L65 L86 G87 A88 S90 Y101 A131 T133 Y157 K161 N186 H187 R192

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008446 GDP-mannose 4,6-dehydratase activity
• GO:0016829 lyase activity
• GO:0070401 NADP+ binding

Biological Process

• GO:0019673 GDP-mannose metabolic process

External links

• PDB: RCSB:7kf3, PDBe:7kf3, PDBj:7kf3
• PDBsum: 7kf3
• UniProt: Q2YMP3