Structure of PDB 7k5b Chain B

Receptor sequence

>7k5bB (length=4524) Species: 5911 (Tetrahymena thermophila)

MGDHSQKDSPEDFIINRLSQALGIQKEKIKKSLETAQDDKGEVTNKDEFQ
GFIQQDNSTNILWVSGQSEKCTFYYGQLPPIDKFKKKGIAIKLGLHKLTN
ENVAKDVVVVLLEHLNSVFNEIMSPVMQNPLNQQGWTDLVAKDLMEKFNN
YVAQVYVLLGQIKGKTMLPLPSHKLTSKDKAHVFEGSIITWTKQIKNVLK
LEPEQLLKYGNDPGPLAEIEFWQNKRDNLNLIDSQLKSVEVQNILHFLKS
TYTTPFTKLQAEVKKARLEANENYRYLFTLKDLFSKLQESQPSDFPTLYE
LFIPIMHTILLIYNKSKTYNQPPRLVVLIREICNAIISNAQAFVDKDTIF
SLIDSKETTEACDKLQVTLDVCSKFKDAYFEYKAKAGGNWKLTSNALFVR
LDSFLERCQDILHLTNTIVQFNKLEKIELGGTKGKTLTESIAQIFKEFEE
AVQAFTSVSYDIMNIAEKKFDDDFYEFRSKIKELERRLASVITQGFDDYD
TIYGRFKLLDNFEGLLTRPIIADELEKKHIVLLEMYKQDLKQVQSIFLEG
KQFVDSMHENAPLFLNMPPIAGALTWCKSLRDRIQEPIEKLAQLGQGITE
REEYKDVQKLYTSITKSIKDYEDQKILSWEKEVEDSSQDKLKQTLLCKDE
NDLIKVNFDPSLVRLLKEVKYFLLLRLEVPTTAKDIYTKAETYRTQIVAL
DMIVDNYNHIKTCLLPVEEPLVKKKIQDMEEEVKPGIEEIRWKSTNIDQF
ISKSKSIVDQLFETVNKMKDSLQKIHKSLANFNVKIIERKNRPMSPDDYD
QFLKAIFSNKLTIVKDNGNQIQKLVKEVLDAVKADKKQNSWKNYNDYVNV
IVIEGISTAIQTALLHLNEQINPVFIKRNDISPLFDIRLELGQSGIQFDP
EIGESSNQLTVRNTIRNWINDFFNIAGTIQRLDTTMPGDFLQEIRSFFEI
KQCLAMITQNLEWIENECNQFRARFDTYSYLWTEDEQISFNRFLDNLDLF
DEKITHLKAIQQEISRIKTPEDISWLRINLQPMKTALDARVTRWIRVYTD
FLVNQFRTTQKNLLDFIEKTKDGIKKNPADHENLHDKKLLMSVMKVISDV
KDVEPRREGIITRMKEMVTKLKKHNVPITEKGTDDPLQQIDNANSNFIEI
YGRVFKVKADIIPLQAEETQNIKRDLDIFMKEVESFRKEFMQKSMGYENI
NNAYDTIMVYYHKLTAIEGRALEYNNLEKAFELQKSNYKQLKDCMNDLKN
LKTMWDAIALIHFQYNDWKTKPWRQIKADILLDTNKTLGTQIKNLPKEIR
NFKGYNVIVEKVKNMGTVLPLVSALHSEFMEDRHWSQLKQITGTVFDHNS
LSFYFEDILALNLYKYENTVNEIVDVAQKEAKIEKKLKNIEQWWSKQVFE
FTEYKETKTFASLDNMMEVLDQHSLDLMGMKSQGKYVEFFYDRVEDWREK
LGRVDVVVNEWLKVQKNWKILYNIFLLSEDIRMQLPEDTKVFEGVDKEFK
DMMSEVSANPSVVEACTIERRDVLVGWSQAIKKCEKALNDYLEQKKKSFP
RFYFLSNQSLLTILSNGQNAPKVCEYLGDCFDGLKTLTFEPPANPAETSK
VGIGMISKDDEKVPFSSKFICEGAVEHWLLNLEFRMRETLQEILEGAKNT
ADLWDSGDKPREEWVEGYNAQIALLTTTIVWTEDVGRAFEDLAGGSETAM
KECQKLIEVRLENLIKKVRGDLHILERWKIINIITIDVHSRDVVEKFVIQ
KVSEAESFAWLSQLKFYWENKPDSDMHLRQTLRFPWEKDKNKNKCIIRIV
DWFRFYSYEYIGNAIRLVITPLTDRCYITLTQALNLTMGGAPAGPAGTGK
TETTKDLGRAIGIPVMVFNCSDQMNKDSMAQIFMGLSQSGAWGCFDEFNR
ISIEVLSVVSTQVKCVLDALKEKKTKFSFVEEGEIQLQDTVGFFITMNPG
YAGRTELPENLKALFRSCAMVVPDLALICENMLMSEGFTMARVLSRKFVS
LYMLSRELLSKQKHYDWGLRAVKSVLRQAGKLKRGDPDMPEDPLLMRALR
DFNMPKIVTDDKVIFRRLIGDLFPKLDPPTKQNPELKKIVQDTTKKDMGL
VAEELFVTKVVQLAEILEVRHCCFVIGPPGSGKTCVWKTLIKSYINSGED
AEYDTLNPKAVTSDELFGAYTKTKEWKNGVIAVIMKNQVKNEEKYKATHM
HKWSVLDGDIDPEWIESLNTVMDDNKVLTLVSNDRIFLTPQMRLIFEISN
LRNATPATVSRAGVLFINETDIGWMPYMNSWLERSQINILKQQKEMANMP
EYPVIDDVAKSVFYRCFQSYFEQNIDVHDKNRVRHICPMVDIAMIQTICT
ILDALLIQHLPKLKQMKEEDEKQALEAFFIFAGLWAIGGPVGGGQDDSKD
MKEFNTVWKGAAKVKFPEQGLCYDYYYDINENKWNTWKVEDYLPNDQPLF
SKIYVATIHTTRLRYMIDIHLQRRKPILFIGSAGTGKTAVVRDYLNSTRP
EQVSHKTINFSSFTDSLALQKNIESMVEKKNGRNYGSATNKVLICFIDDF
NMPYVDKYGTQSPIQLLRLILDYGSIFNREQLEERKFLQDLLFFGCLNQK
SGSFTVDLRLQRNFSVFSMYTPSSDVIKTIFGSILNAHLSTIDDKAQKMA
FKLVEATYFTFDKILKNTTAFAPSAKRFHYQFNFRELARVCEGICRTTPG
QYSGGDQGKLVRLWAHEMKRTFEDRFIANEHVEFFRRYLTEAISKCIGEF
PETENPIAEPLIFTGFVAAHQGLDQQYTQCTIPVLKRVLDDKLEEYNEVK
AQMNLVLFQQAMEHVSRICRILDMPGNNALLVGVGGSGKQSLCRLSTFIN
GFEIDQLVVTASFTINDLRNNLQEIYKKIAKPNSIARVFMITDSQIKEEQ
FLIPINDMLNSGWIFDLFPKEDMDSLVSGVRNEAKGEGVDVNNLTALTSY
FLDKIRKNLKVVLCFSPVGDTMRIRSRKFPGIINNTSIDWFHPWPHEALI
DVAFRFLEEIEFPTEEIRQSISLNMAKVHSSIDTANEKFLKLERRYNYTT
PKSFLELIDFYKKLLTEKRETIQRQIQRYEMGLNILAETQNKVQGLQEEL
KVKMVEVNKQREETDILIEKVGKESALAEEEQTIANAEEEKTNVAAAEAE
KISKEATEALAEALPALRSAEAAVDCLKKPHVTEMKNLGSPPAGVIVTAR
VVLILFNQGITLNDPDEKVWKKAVTFMNNPQAFIDKVKSFDGENIEPNII
EQSNKIIQDPSKKFNEKDMAGQSYAASKLCAWAVNIVTFNKIFKQVKPLQ
DAQKQANEILEEKKKELAIVKQRVAELNARVNSLKRQLEEAEARKMIVEQ
DAARCQSRLSAAENLVNGLAGENKRWTQNVKFLKENIKSMIGDSLLASAF
VSYIGAFSAKLRLELWKNTWLPDIIEKGIPITEGIEPLKILTTEAIKSKW
KNEGLPADPMSLENAAIITACARWPLIIDPQLQGSTWIRGKQGENLTTIS
LSQPKWLGALTSSISSGRAVLIEGIQQEIDATLDPLLQRAVKKNGNQLQL
EIGGDPIDYDPNFKLFLMTKLINPHFRPEIAAQCTIINFIVTESGLEEQF
IAMVVNIEKNELEMAKQDLVKKQNEYAVTLDKLESDLLQSLSEADPATIL
DNTELIQNLDKTKKTTIEITEQQQKAKVTEAEINIQREHYRVVAAEGSML
YFLVISLSVMDHMYQYSLESFITFFFKAINRTTVRDENRIPTLILNIRQT
IYQWISRGLFEKHKLIFLTLIVFRLMQKKIIDVAYEVAEMDFLIKCPARP
GVENTLDWLPNISWDQIQGLINLEEFRNFAHQLEKEAPNRFKDWYNELQP
EDQKLPLDWKRLDSMPFKKLLVLRCLRPDRMTISLNNFIRAVLPQGDAFV
EMDQKLAFSEILESVINEDAESTIPIFFILSPGSDPVKEVEKIAKKKRIE
PGKNFFNIALGQGQDEIARRRIEEGNKEGHWVMLQNIHLMPTWLIELEKI
LDSYSGEAGNSEFRLFLSAEPSTGIPIGILDRSIKLTNEPPAGLKANMKR
AWTYFSKEEIEDKDPKIKSILFALCFFHSTLIERRRFGPKGWNMSYPFNM
GDLRDSYLVMNRYMEQNQGGKVPFNDLIYIFGEIMYGGHIVDDWDRRLCN
SYLFNTMHEQLFDELELFPYIEGKGLSFKVPGQNPYEKYIEHIETSLKQE
TPLAYGLHPNAEIGFRTDQCKTLFNTLLELMPKEQSRDIKSSNEMASDLI
KQLLEDSELKNKIFNMEEIKNKIDAENKGPYQNVFLQEIEYMNALLSEIV
KDLEEIGQGLSGLLTVSENMEMIIESIALSRVPASWQKLAYPSKRGLQSW
LANLFQRIEQLNIFRDDPYSIPRVVMISRFFNPQSFLTAIMQVISRAKAY
ELNKLYIQTEITKRSIEEIEGAAKEGAYVYGFILEGARWDYQLGQLEESK
PKEMFSVLPVTYCKAIPLPPEGKEDKSLYQCPVYKTEDRGNTYVFTAQLK
TRFPPRKWILAGVAIIMDVEGVSD

3D structure

• PDB: 7k5b Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism.
• Chain: B
• Resolution: 4.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	B	I2510 V2512 A2540 G2543 K2544 T2545 A2546 R2742	I2453 V2455 A2483 G2486 K2487 T2488 A2489 R2685
BS02	ATP	B	L2157 P2186 G2187 S2188 G2189 K2190 T2191 C2192 E2304 P2333 Y2334 I2399 R2666	L2100 P2129 G2130 S2131 G2132 K2133 T2134 C2135 E2247 P2276 Y2277 I2342 R2609
BS03	ADP	B	L2862 V2863 G2893 G2895 K2896 Q2897 S2898 W3051 L3112	L2805 V2806 G2836 G2838 K2839 Q2840 S2841 W2994 L3055

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005858 axonemal dynein complex
• GO:0005874 microtubule
• GO:0005929 cilium
• GO:0030286 dynein complex

External links

• PDB: RCSB:7k5b, PDBe:7k5b, PDBj:7k5b
• PDBsum: 7k5b
• PubMed: 34556869
• UniProt: I7M9J2