Structure of PDB 7k58 Chain B

Receptor sequence

>7k58B (length=4516) Species: 5911 (Tetrahymena thermophila)

MGDHSQKDSPEDFIINRLSQALGIQKEKIKKSLETQQDDKGEVTNKDEFI
QQDNSTNILWVSGQSEKCTFYYGQLIDKFKKKGIAIKLGLHKLTNENVAK
DVVVVLLEHLNSVFNEIMSPVMQNPLNQQGWTDLVAKDLMEKFNNYVAQV
YVLLGQIKGKTMLPLPSHKLTSKDKAHVFEGSIITWTKQIKNVLKLEPEQ
LLKYGNDPGPLAEIEFWQNKRDNLNLIDSQLKSVEVQNILHFLKSTYTTP
FTKLQAEVKKARLEANENYRYLFTLKDLFSKLQESQPSDFPTLYELFIPI
MHTILLIYNKSKTYNQPPRLVVLIREICNAIISNAQAFVDKDTIFSLIDS
KETTEACDKLQVTLDVCSKFKDAYFEYKAKAGGNWKLTSNALFVRLDSFL
ERCQDILHLTNTIVQFNKLEKIELGGTKGKTLTESIAQIFKEFEEAVQAF
TSVSYDIMNIAEKKFDDDFYEFRSKIKELERRLASVITQGFDDYDTIYGR
FKLLDNFEGLLTRPIIADELEKKHIVLLEMYKQDLKQVQSIFLEGKQFVD
SMHENAPLFLNMPPIAGALTWCKSLRDRIQEPIEKLAQLGQGITEREEYK
DVQKLYTSITKSIKDYEDQKILSWEKEVEDSSQDKLKQTLLCKDENDLIK
VNFDPSLVRLLKEVKYFLLLRLEVPTTAKDIYTKAETYRTQIVALDMIVD
NYNHIKTCLLEPLVKKKIQDMEEEVKPGIEEIRWKSTNIDQFISKSKSIV
DQLFETVNKMKDSLQKIHKSLANFNVKIIERKNRPMSPDDYDQFLKAIFS
NKLTIVKDNGNQIQKLVKEVLDAVKADKKQNSWKNYNDYVNVIVIEGIST
AIQTALLHLNEQINPVFIKRNDISPLFDIRLELGQSGIQFDPEIGESSNQ
LTVRNTIRNWINDFFNIAGTIQRLDTTMPGDFLQEIRSFFEIKQCLAMIT
QNLEWIENECNQFRARFDTYSYLWTEDEQISFNRFLDNLDLFDEKITHLK
AIQQEISRIKTPEDISWLRINLQPMKTALDARVTRWIRVYTDFLVNQFRT
TQKNLLDFIEKTKDGIKKNPADHENLHDKKLLMSVMKVISDVKDVEPRRE
GIITRMKEMVTKLKKHNVPITEKGTDDPLQQIDNANSNFIEIYGRVFKVK
ADIIPLQAEETQNIKRDLDIFMKEVESFRKEFMQKSMGYENINNAYDTIM
VYYHKLTAIEGRALEYNNLEKLFELQKSNYKQLKDCMNDLKNLKTMWDAI
ALIHFQYNDWKTKPWRQIKADILLDTNKTLGTQIKNLPKEIRNFKGYNVI
VEKVKNMGTVLPLVSALHSEFMEDRHWSQLKQITGTVFDHNSLSFYFEDI
LALNLYKYENTVNEIVDVAQKEAKIEKKLKNIEQWWSKQVFEFTEYKETK
TFASLDNMMEVLDQHSLDLMGMKSQGKYVEFFYDRVEDWREKLGRVDVVV
NEWLKVQKNWKILYNIFLLSEDIRMQLPEDTKVFEGVDKEFKDMMSEVSA
NPSVVEACTIERRDVLVGWSQAIKKCEKALNDYLEQKKKSFPRFYFLSNQ
SLLTILSNGQNAPKVCEYLGDCFDGLKTLTFEPPANPAETSKVGIGMISK
DDEKVPFSSKFICEGAVEHWLLNLEFRMRETLQEILEGAKNTADLWDSGD
KPREEWVEGYNAQIALLTTTIVWTEDVGRAFEDLAGGSETAMKECQKLIE
VRLENLIKKVRGDLHILERWKIINIITIDVHSRDVVEKFVIQKVSEAESF
AWLSQLKFYWENKPDSDMHLRQTLRFPWEKDKNKNKCIIRIVDWFRFYSY
EYIGNAIRLVITPLTDRCYITLTQALNLTMGGAPAGPAGTGKTETTKDLG
RAIGIPVMVFNCSDQMNKDSMAQIFMGLSQSGAWGCFDEFNRISIEVLSV
VSTQVKCVLDALKEKKTKFSFVEEGEIQLQDTVGFFITMNPGYAGRTELP
ENLKALFRSCAMVVPDLALICENMLMSEGFTMARVLSRKFVSLYMLSREL
LSKQKHYDWGLRAVKSVLRQAGKLKRGDPDMPEDPLLMRALRDFNMPKIV
TDDKVIFRRLIGDLFPKLDPPTKQNPELKKIVQDTTKKDMGLVAEELFVT
KVVQLAEILEVRHCCFVIGPPGSGKTCVWKTLIKSYINSGEDAEYDTLNP
KAVTSDELFGAYTKTKEWKNGVIAVIMKNQVKNEEKYKATHMHKWSVLDG
DIDPEWIESLNTVMDDNKVLTLVSNDRIFLTPQMRLIFEISNLRNATPAT
VSRAGVLFINETDIGWMPYMNSWLERSQINILKQQKEMANMPEYPVIDDV
AKSVFYRCFQSYFEQNIDVHDKNRVRHICPMVDIAMIQTICTILDALLIQ
HLPKLKQMKEEDEKQALEAFFIFAGLWAIGGPVGGGQDDSKDMKEFNTVW
KGAAKVKFPEQGLCYDYYYDINENKWNTWKVEDYLPNDQPLFSKIYVATI
HTTRLRYMIDIHLQRRKPILFIGSAGTGKTAVVRDYLNSTRPEQVSHKTI
NFSSFTDSLALQKNIESMVEKKNGRNYGSATNKVLICFIDDFNMPYVDKY
GTQSPIQLLRLILDYGSIFNREQLEERKFLQDLLFFGCLNQKSGSFTVDL
RLQRNFSVFSMYTPSSDVIKTIFGSILNAHLSTIDDKAQKMAFKLVEATY
FTFDKILKNTTAFAPSAKRFHYQFNFRELARVCEGICRTTPGQYSGGDQG
KLVRLWAHEMKRTFEDRFIANEHVEFFRRYLTEAISKCIGEFPETENPIA
EPLIFTGFVAAHQGLDQQYTQCTIPVLKRVLDDKLEEYNEVKAQMNLVLF
QQAMEHVSRICRILDMPGNNALLVGVGGSGKQSLCRLSTFINGFEIDQLV
VTASFTINDLRNNLQEIYKKIAKPNSIARVFMITDSQIKEEQFLIPINDM
LNSGWIFDLFPKEDMDSLVSGVRNEAKGEGVDVNNLTALTSYFLDKIRKN
LKVVLCFSPVGDTMRIRSRKFPGIINNTSIDWFHPWPHEALIDVAFRFLE
EIEFPTEEIRQSISLNMAKVHSSIDTANEKFLKLERRYNYTTPKSFLELI
DFYKKLLTEKRETIQRQIQRYEMGLNILAETQNKVQGLQEELKVKMVEVN
KQREETDILIEKVGKESALAEEEQTIANAEEEKTNVAAAEAEKISKEATE
ALAEALPALRSAEAAVDCLKKPHVTEMKNLGSPPAGVIVTARVVLILFNQ
GITLNDPDEKVWKKAVTFMNNPQAFIDKVKSFDGENIEPNIIEQSNKIIQ
DPSKKFNEKDMAGQSYAASKLCAWAVNIVTFNKIFKQVKPLQDAQKQANE
ILEEKKKELAIVKQRVAELNARVNSLKRQLEEAEARKMIVEQDAARCQSR
LSAAENLVNGLAGENKRWTQNVKFLKENIKSMIGDSLLASAFVSYIGAFS
AKLRLELWKNTWLPDIIEKGIPITEGIEPLKILTTEAIKSKWKNEGLPAD
PMSLENAAIITACARWPLIIDPQLQGSTWIRGKQGENLTTISLSQPKWLG
ALTSSISSGRAVLIEGIQQEIDATLDPLLQRAVKKNGNQLQLEIGGDPID
YDPNFKLFLMTKLINPHFRPEIAAQCTIINFIVTESGLEEQFIAMVVNIE
KNELEMAKQDLVKKQNEYAVTLDKLESDLLQSLSEADPATILDNTELIQN
LDKTKKTTIEITEQQQKAKVTEAEINIQREHYRVVAAEGSMLYFLVISLS
VMDHMYQYSLESFITFFFKAINRTTVRDENRIPTLILNIRQTIYQWISRG
LFEKHKLIFLTLIVFRLMQKKIIDVAYEVAEMDFLIKCPARPGVENTLDW
LPNISWDQIQGLINLEEFRNFAHQLEKEAPNRFKDWYNELQPEDQKLPLD
WKRLDSMPFKKLLVLRCLRPDRMTISLNNFIRAVLPQGDAFVEMDQKLAF
SEILESVINEDSESTIPIFFILSPGSDPVKEVEKIAKKKRIEPGKNFFNI
ALGQGQDEIARRRIEEGNKEGHWVMLQNIHLMPTWLIELEKILDSYSGEA
GNSEFRLFLSAEPSTGIPIGILDRSIKLTNEPPAGLKANMKRAWTYFSKE
EIEDKDPKIKSILFALCFFHSTLIERRRFGPKGWNMSYPFNMGDLRDSYL
VMNRYMEQNQGGKVPFNDLIYIFGEIMYGGHIVDDWDRRLCNSYLFNTMH
EQLFDELELFPYIEGKGLSFKVPGQNPYEKYIEHIETSLKQETPLAYGLH
PNAEIGFRTDQCKTLFNTLLELMPKEQSRDIKSSNEMASDLIKQLLEDSE
LKNKIFNMEEIKNKIDAENKGPYQNVFLQEIEYMNALLSEIVKDLEEIGQ
GLSGLLTVSENMEMIIESIALSRVPASWQKLAYPSKRGLQSWLANLFQRI
EQLNIFRDDPYSIPRVVMISRFFNPQSFLTAIMQVISRAKAYELNKLYIQ
TEITKRSIEEIEGAAKEGAYVYGFILEGARWDYQLGQLEESKPKEMFSVL
PVTYCKAIPLPPEGKEDKSLYQCPVYKTEDRGNTYVFTAQLKTRFPPRKW
ILAGVAIIMDVEGVSD

3D structure

• PDB: 7k58 Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism.
• Chain: B
• Resolution: 4.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	B	L2157 V2158 F2163 P2186 S2188 G2189 K2190 T2191 C2192 E2304 P2333 Y2334 I2399 R2666 R2669	L2092 V2093 F2098 P2121 S2123 G2124 K2125 T2126 C2127 E2239 P2268 Y2269 I2334 R2601 R2604
BS02	ADP	B	I2510 V2512 A2540 G2541 G2543 K2544 T2545 A2546 I2687 R2742	I2445 V2447 A2475 G2476 G2478 K2479 T2480 A2481 I2622 R2677
BS03	ADP	B	L2862 V2863 F2865 G2893 S2894 G2895 Q2897 S2898 W3051	L2797 V2798 F2800 G2828 S2829 G2830 Q2832 S2833 W2986

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005858 axonemal dynein complex
• GO:0005874 microtubule
• GO:0005929 cilium
• GO:0030286 dynein complex

External links

• PDB: RCSB:7k58, PDBe:7k58, PDBj:7k58
• PDBsum: 7k58
• PubMed: 34556869
• UniProt: I7M9J2