Structure of PDB 7fe1 Chain B

Receptor sequence

>7fe1B (length=712) Species: 1169312 (Enterococcus faecalis ATCC 10100)

MNIQAIDTRHGTANQHSFSNGNCLPYTGVPFGMNFYAPQTTDQKGSWWFH
PEDRTFQGYRVTHQPSPWMGDFSHLLMTPVSGSLSELSLFHAQSSYRPEE
SLFSPVEINLTQLRYQITSQLIPSMYGGILTIDYQQKDNHLLLTLPGRYQ
VKQLDDHQVAVKVINYSGCEDPDFSFYFVLHFEQPLTKWFAPSSGEDGKI
LLSFGNIAQQVVHFSSSFISEKQAQLNLAREISLRSTEMLQQGIADWHNY
FDRLKVTHENPEHTKTFYHTLYRTFLFPQTFYELDENQQPIHYDTFSQTV
RPGVLYTNNGFWDTYKTVYPLFSLIAQEKYEEMLEGFLNSYNETGFLPKW
LSPDERGLMPGTLIDAVIADAAVKKIRPDLMPQFLEAMKKGATQQSEREN
YGRQGTLDYLKYGYVPSTYHESVNHTLDYAYSDFCISQVAKTLNDSETAT
FYRQQALNYQQLFNPETGFMQAKDTEGNFRPDFLDIRWGKDYAEGSAWQS
SFAVYQDFAGLIKLYGSELAFEKKLIQLCNQAPNFNVEGYGFEIHEMSEM
AAIDFGQLAISNQPSFHYPFLFSYIGKPEMAQPLLKQLMQTFDASPTGYP
GDEDNGSMSAWYIFNSLGFYPVTPGAGEYVIGMPLVQTAEVKLSNGKQLT
IQTSPNKVQQQFIHEIQLNQEKHTAPYFTHQELLNGGTLDYQLGIVPNPQ
TTAERPFSLSTE

3D structure

• PDB: 7fe1 Unlocking the Hydrolytic Mechanism of GH92 alpha-1,2-Mannosidases: Computation Inspires the use of C-Glycosides as Michaelis Complex Mimics.
• Chain: B
• Resolution: 1.72 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	5II	B	W68 Y540 H545 E546	W68 Y540 H545 E546
BS02	MAN	B	S66 W68 D313 W350 L358 M359 D604	S66 W68 D313 W350 L358 M359 D604
BS03	CA	B	N562 Q563 D604	N562 Q563 D604

Gene Ontology

Molecular Function

• GO:0000224 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity
• GO:0003824 catalytic activity
• GO:0030246 carbohydrate binding
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
• GO:0006516 glycoprotein catabolic process
• GO:0006517 protein deglycosylation

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:7fe1, PDBe:7fe1, PDBj:7fe1
• PDBsum: 7fe1
• PubMed: 35049087
• UniProt: Q832K9