Structure of PDB 7f99 Chain B

Receptor sequence

>7f99B (length=488) Species: 9606 (Homo sapiens)

GLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFF
DAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAE
PIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRT
REFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKE
KFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGE
KQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNA
LSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVP
IRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFT
RASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARM
GAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

3D structure

• PDB: 7f99 Targeting prolyl-tRNA synthetase via a series of ATP-mimetics to accelerate drug discovery against toxoplasmosis.
• Chain: B
• Resolution: 1.979 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.15: proline--tRNA ligase.
  6.1.1.17: glutamate--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	1UI	B	R1152 E1154 K1156 H1157 P1158 L1162 R1163 T1164 R1165 F1167 W1169 Q1237 G1239 T1240 T1276 R1278	R138 E140 K142 H143 P144 L148 R149 T150 R151 F153 W155 Q223 G225 T226 T262 R264
BS02	ZN	B	C1448 C1453 C1495 C1497	C434 C439 C471 C473
BS03	CA	B	D1096 E1100	D82 E86
BS04	PRO	B	T1121 E1123 W1169 E1171 H1173 F1216 H1242 S1272 W1273 G1274	T107 E109 W155 E157 H159 F202 H228 S258 W259 G260

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004827 proline-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006433 prolyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7f99, PDBe:7f99, PDBj:7f99
• PDBsum: 7f99
• PubMed: 36854028
• UniProt: P07814|SYEP_HUMAN Bifunctional glutamate/proline--tRNA ligase (Gene Name=EPRS1)