Structure of PDB 7ea9 Chain B

Receptor sequence

>7ea9B (length=500) Species: 9606 (Homo sapiens)

DPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHL
TDITLKVAGRIHAKRASGGKLIFHDLRGEGVKLQVMANSRNYKSEEEFIH
INNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLKDKET
RYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIP
GGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNE
GIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHP
DGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDD
ICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKW
HRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDE
AMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKP

3D structure

• PDB: 7ea9 Structural analyses of a human lysyl-tRNA synthetase mutant associated with autosomal recessive nonsyndromic hearing impairment.
• Chain: B
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-
  6.1.1.6: lysine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	KAA	B	E301 R323 H331 N332 F335 E339 Y341 E494 I495 N497 Y499 E501 G546 G548 G550 R553	E226 R248 H256 N257 F260 E264 Y266 E419 I420 N422 Y424 E426 G471 G473 G475 R478

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004824 lysine-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006430 lysyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7ea9, PDBe:7ea9, PDBj:7ea9
• PDBsum: 7ea9
• PubMed: 33784510
• UniProt: Q15046|SYK_HUMAN Lysine--tRNA ligase (Gene Name=KARS1)