Structure of PDB 7c7l Chain B

Receptor sequence

>7c7lB (length=372) Species: 115547 (uncultured archaeon)

TITKTLKLRIVRPFWQEISEIFRQLQKQAAEIYNQSLIELYYEIFIKGKG
IANASSVEHYLSDVCYTRAAELFKNAAIASGLRSKIKSNFRLKELKNMKS
GLPTTKSDNFPIPLVFEISNFIIKIPFGRWQVKKEIDKYRPWEKFDFEQV
QKSPKPISLLLSYIEVKRGSSAWMLNLSIDVPDPSIIGGIAVGVKSPLVC
AINNAFSRYSISDNDLFHFNKKMFARRRILLKKPITILTEKSERFRKKLI
ERWACEIADFFIKNKVGTVQMENLESMKRKEDSYFNIRLRGFWPYAEMQN
KIEFKLKQYGIEIRKVAPNNTSKTCSKCGHLNNYFNFEYRKKNKFPHFKC
EKCNFKENADYNAALNISNPKL

3D structure

• PDB: 7c7l Structure of the miniature type V-F CRISPR-Cas effector enzyme.
• Chain: B
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	E138 K167 K186 K330 F341 D348 F352 H353 K356 K357 F359 A360 R361 R363 I364 K391 R394 F395 R438	E58 K87 K106 K195 F206 D213 F217 H218 K221 K222 F224 A225 R226 R228 I229 K241 R244 F245 R288
BS02	dna	B	T9 K11 N305 F341 R343 Y344	T5 K7 N176 F206 R208 Y209
BS03	dna	B	Y232 P234 K237	Y139 P141 K144
BS04	ZN	B	C475 C478 C500 C503	C325 C328 C350 C353

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:7c7l, PDBe:7c7l, PDBj:7c7l
• PDBsum: 7c7l
• PubMed: 33333018
• UniProt: A0A482D308|CS12F_UNCAX CRISPR-associated endodeoxyribonuclease Cas12f1 (Gene Name=cas12f)