Structure of PDB 7bcw Chain B

Receptor sequence
>7bcwB (length=574) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
DLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFMLSLLKPLLDDG
FGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRRRLF
GHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIG
LFIMMFYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTT
SAEQMLKGHKEVLIFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPII
QLIASLALAFVLYAASFPSVMDSLTAGTITVVFSSMIALMRPLKSLTNVN
AQFQRGMAACQTLFTILDSEQEKDEGKRVIERATGDVEFRNVTFTYPGRD
VPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRFYDIDEGEILMDGH
DLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQIEEAAR
MAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILD
EATSALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGV
IVERGTHNDLLEHRGVYAQLHKMQ
3D structure
PDB7bcw Cryo-EM structure of MsbA in saposin-lipid nanoparticles (Salipro) provides insights into nucleotide coordination.
ChainB
Resolution3.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 7.5.2.6: ABC-type lipid A-core oligosaccharide transporter.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 VO4 B S482 G483 G484 A510 S477 G478 G479 A505
BS02 ADP B L480 S482 Q485 L475 S477 Q480
BS03 VO4 B S378 K382 Q424 E506 H537 S373 K377 Q419 E501 H532
BS04 ADP B D117 Y351 S380 K382 S383 D112 Y346 S375 K377 S378
BS05 MG B S383 Q424 S378 Q419
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0008289 lipid binding
GO:0008559 ABC-type xenobiotic transporter activity
GO:0015437 lipopolysaccharide floppase activity
GO:0016887 ATP hydrolysis activity
GO:0034040 ATPase-coupled lipid transmembrane transporter activity
GO:0042802 identical protein binding
GO:0140359 ABC-type transporter activity
Biological Process
GO:0006869 lipid transport
GO:0015920 lipopolysaccharide transport
GO:0034204 lipid translocation
GO:0042908 xenobiotic transport
GO:0055085 transmembrane transport
Cellular Component
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0043190 ATP-binding cassette (ABC) transporter complex
GO:1990199 MsbA transporter complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7bcw, PDBe:7bcw, PDBj:7bcw
PDBsum7bcw
PubMed34921499
UniProtP60752|MSBA_ECOLI ATP-dependent lipid A-core flippase (Gene Name=msbA)

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