Structure of PDB 7b0y Chain B

Receptor sequence

>7b0yB (length=1131) Species: 9825 (Sus scrofa domesticus)

DEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPP
IDLQAPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLY
VDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELN
ECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCL
ENSSRPTSTIWVSMLARGAIGQRIVATLPYIKQEVPIIIVFRALGFVSDR
DILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTK
EKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRREL
DDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLE
LAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHL
RRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYI
SVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQ
LMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQK
LLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDL
QEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQA
MGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAI
ASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKP
TRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPRYTKRDCS
TFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKG
TCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANK
GEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFI
GPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDC
QIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNK
TQISLVRMPYACKLLFQELMSMSIAPRMMSV

3D structure

• PDB: 7b0y Structure of a transcribing RNA polymerase II-U1 snRNP complex.
• Chain: B
• Resolution: 3.6 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	P231 T232 S233 Y262 K264 K327 N410 K457 K458 H460 Q461 D492	P206 T207 S208 Y230 K232 K295 N378 K425 K426 H428 Q429 D460
BS02	rna	B	A464 G465 Q468 A727 Q731 G839 M840 R841 H842 K942 H1053	A432 G433 Q436 A695 Q699 G807 M808 R809 H810 K899 H1010
BS03	dna	B	N188 R438 T746 G1077 R1078 R1085	N163 R406 T714 G1034 R1035 R1042
BS04	ZN	B	C1119 C1122 C1137 C1140	C1076 C1079 C1094 C1097

Gene Ontology

Molecular Function

• GO:0001055 RNA polymerase II activity
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006366 transcription by RNA polymerase II

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005654 nucleoplasm
• GO:0005665 RNA polymerase II, core complex

External links

• PDB: RCSB:7b0y, PDBe:7b0y, PDBj:7b0y
• PDBsum: 7b0y
• PubMed: 33446560
• UniProt: A0A0B8RVL1