Structure of PDB 7aud Chain B

Receptor sequence

>7audB (length=513) Species: 9606 (Homo sapiens)

NLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFI
LMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYL
TGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERK
LLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRV
QKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHP
YDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQ
DGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEIS
HCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECR
RIQLLAYFGENGFNPDFCKKHPDVSCDNCCSQREEMVKKCLGELTEVCKS
LGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEV
ISVLQKYSEWTSP

3D structure

• PDB: 7aud Uncovering an allosteric mode of action for a selective inhibitor of human Bloom syndrome protein.
• Chain: B
• Resolution: 2.96 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	R717 T738 G739 T766 E768 K769 S773 N774 R775 F807 R808 L896 S897 R898 H919 A920 T946 I947 K968 R1003 M1007 E1008	R80 T101 G102 T129 E131 K132 S136 N137 R138 F170 R171 L259 S260 R261 H282 A283 T309 I310 K331 R366 M370 E371
BS02	ZN	B	C1036 C1055 C1063 C1066	C399 C418 C426 C429
BS03	ADP	B	L665 N667 R669 Q672 T691 G692 G694 K695 S696	L28 N30 R32 Q35 T54 G55 G57 K58 S59
BS04	RY8	B	S801 Q802 H805 D840 Q975 T1015 T1018 N1022	S164 Q165 H168 D203 Q338 T378 T381 N385

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006310 DNA recombination
• GO:0044237 cellular metabolic process

External links

• PDB: RCSB:7aud, PDBe:7aud, PDBj:7aud
• PDBsum: 7aud
• PubMed: 33647232
• UniProt: P54132|BLM_HUMAN RecQ-like DNA helicase BLM (Gene Name=BLM)