Structure of PDB 7aoe Chain B

Receptor sequence

>7aoeB (length=1174) Species: 284812 (Schizosaccharomyces pombe 972h-)

MSFQTLERERTFKNPPKDGTSFPDLQKAVKPHVDSFNALTNAGLLNYAVK
EIGEKCAFDSITQEEGGALKFGNKISFRVDEVQIAKPMLSSRERSSINRK
VYPAEARERLTTYKSRLVLKFSWSVNGGPRQSEMREVGMIPIMVRSNRCH
LEGLSPAELIAHKEESEEMGGYFIVNGIEKLIRMLILPKRNHPTAIIRPS
FANRGTSYSQYGLSIRCVRPDQSSLTNTLHYLNNGVTMFRFHWRKNEYLI
PSMMILKALLETSDKEIFEGIVGKDLGNTFLTDRVELMLRAYKSYGLYSQ
TETLQYLGSKFRVVLGVAEDLTDVEVGRFLLQKVVLVHLREAKDKFRLLL
FMIRKLYALVAGECCADNPDSPQHQEILLGGFLYGQILKEKIEDWLNSIR
AQINLDVRRSAPGVDFSDRKYLTRVFSKINNDIGTKLQYFLSTGNLVSNT
GLDLQQATGYTVVAEKLNFYRFLSHFRMVHRGAFFAELKTTTVRKLLPEA
WGFMCPVHTPDGSPCGLLNHLARKCEIVTHPSDVSQIPSLLLSLGVDPPS
VVGHESGWGCVQLDGKIVGWCTYKLAKHVADVLRLMKIEYAVKLRNGTAT
EPAKVPLDLEIGYVPPSHNGQYPGLYLFSNPARMVRPVKHISTGELDMLG
PFEQVYMDIACFPKEIVPKVSTHVEYSPTNVLSIVANMTPFSDFNQSPRN
MYQCQMGKQTMGTPGTALRYRTDNKLYRLQTGQTPVVRPKLHNTYGLDHY
PNGTNAVVAVISYTGYDMEDAMILNKSAHERGFGYGTVYKGESFDLSQKR
RRGEPVVHHFGFAPGSTPRREWLQKLDADGLPFIGIKLEDGDPIVAYYDE
STGQNFIETYHGTEPGFVDEVRLLGNDVGDSECQQIHVKLRITRSPIIGD
KFSSRHGQKGICSQKWPTVDMPFTESGMQPDIIINPHAFPSRMTIGMFIE
SLAGKAGACHGLAQDSTPFIYSEQQTAADYFGEQLVKAGYNYHGNEPMYS
GITGQEMKADIYIGVVYYQRLRHMVSDKFQVRTTGPIHNLTRQPVKGRKR
AGGIRFGEMERDAVIGHGTSFLMQDRLMNCSDYAQSWVCRDCGSIISIMS
TISMNGVGSASEVRCRSCAKPALGLEDTSDIWQDGSGKKFVGGTNTTLIA
LPSVFNYLTAELTAMNIKMMLEVK

3D structure

• PDB: 7aoe Conserved strategies of RNA polymerase I hibernation and activation.
• Chain: B
• Resolution: 3.9 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	I178 S442 N724 Q1030 K1046 G1047 R1048 R1055	I178 S442 N724 Q1030 K1046 G1047 R1048 R1055
BS02	rna	B	Q705 Q709	Q705 Q709
BS03	ZN	B	C1089 C1092 C1115 C1118	C1089 C1092 C1115 C1118

Gene Ontology

Molecular Function

• GO:0001054 RNA polymerase I activity
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006362 transcription elongation by RNA polymerase I

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005736 RNA polymerase I complex

External links

• PDB: RCSB:7aoe, PDBe:7aoe, PDBj:7aoe
• PDBsum: 7aoe
• PubMed: 33536435
• UniProt: Q9P7X8|RPA2_SCHPO Probable DNA-directed RNA polymerase I subunit RPA2 (Gene Name=rpa2)