Structure of PDB 6wte Chain B

Receptor sequence

>6wteB (length=530) Species: 452652 (Kitasatospora setae KM-6054)

MSRLVLLVNPNKVHPPIAPYALDVLTTALEDEGFEVEVLDLTFRRDDWKT
CLHEYFAERSPMLVGVTVRNTDTVYAFEQRPFVGEHREIITEIRRLTDAP
VVGGGIGFSTMPFALVEYFGIEYGVKGPGEKILCELATAISEGRDTAGIP
GLIRNTERGAVRVPPPAEPTGQFEPRVWQVDQLSVYRRRSGVPRKVDNLE
YYRRGGLGSILTKNGCAYRCSHCVEPDAKGTRYGQRELASVVDEMESLAA
QGILDQHTTDSEFNLSIAHAKNLLREIVRRRHADPDNPLNRLRLWVYCQP
SPFDEEFADLLAAAGCRGVNVGSDHIRPELLSGWKVTEKGGTYYTFEDTE
RLVRLCRERGILTMVEALFGMPGETPETVRACVDAFMALDATVTGFSLGL
RLFPYTPMGIEIAEQCAGVRTAPGLQSNTADGPIVLKPLRMCASPAEYER
QFMFDEHGNFRLVCYFSPGLLWHGAVADLWALIDPADHHRVMLPTVNPFL
TSLGGLGYTGAFWSHWRGREEIMRKAREAA

3D structure

• PDB: 6wte Structural basis for non-radical catalysis by TsrM, a radical SAM methylase.
• Chain: B
• Resolution: 1.67 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	B	C227 Y229 C231 C234 P237 E273 Q310 Y354	C216 Y218 C220 C223 P226 E262 Q299 Y343
BS02	B12	B	N11 V13 I17 A21 L25 T67 R69 N70 T71 V74 Y75 G104 G105 I106 V125 G127 P128 G129 E130 L218 E236 K240 T270 F414	N11 V13 I17 A21 L25 T67 R69 N70 T71 V74 Y75 G104 G105 I106 V125 G127 P128 G129 E130 L207 E225 K229 T259 F403

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0031419 cobalamin binding
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:6wte, PDBe:6wte, PDBj:6wte
• PDBsum: 6wte
• PubMed: 33462497
• UniProt: E4N8S5