Structure of PDB 6w8v Chain B

Receptor sequence

>6w8vB (length=825) Species: 10090 (Mus musculus) [Search protein sequence]

DRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLAR
VTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYI
HSKVKVIYKAPSENWAMEGGTYFFQLWYNQEYARFESPPKTQPTEDNKHK
FCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYL
PPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPY
RIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINML
YWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSK
TKNFEDPPNHARSPGNKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIE
MWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVE
MLCGGPPCQGFSGMNRFNSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFV
SYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGE
KLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMS
DLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVA
ARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGAL
RGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFF
STTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRH
RQVGNAVPPPLAKAIGLEIKLCLLS

3D structure

PDB

6w8v DNMT1 activity, base flipping mechanism and genome-wide DNA methylation are regulated by the DNA sequence context

Chain

Resolution

3.12 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	C1229 E1269 R1313 R1315
Catalytic site (residue number reindexed from 1)	C458 E494 R538 R540
Enzyme Commision number	2.1.1.37: DNA (cytosine-5-)-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	B	G1234 R1237 Q1343 R1427 R1492 C1501 H1504 R1508 W1512 E1533 M1535 K1537	G463 R466 Q568 R652 R717 C726 H729 R733 W737 E758 M760 K762
BS02	dna	B	S981 Y983 K985 G1226 P1227 P1228 C1229 Q1230 S1233 M1235 E1269 V1271 R1313 R1314 R1315 N1510 T1527 T1530 G1536 K1537	S236 Y238 K240 G455 P456 P457 C458 Q459 S462 M464 E494 V496 R538 R539 R540 N735 T752 T755 G761 K762
BS03	SAH	B	F1148 S1149 G1150 G1153 L1154 E1171 M1172 N1580 A1581 V1582	F377 S378 G379 G382 L383 E400 M401 N805 A806 V807
BS04	ZN	B	C1479 C1481 C1487 H1504	C704 C706 C712 H729
BS05	ZN	B	H796 C823 C897	H65 C92 C152

Gene Ontology

	Molecular Function
GO:0003682	chromatin binding
GO:0008168	methyltransferase activity

View graph for
Molecular Function

External links

PDB	RCSB:6w8v, PDBe:6w8v, PDBj:6w8v
PDBsum	6w8v
PubMed
UniProt	P13864\|DNMT1_MOUSE DNA (cytosine-5)-methyltransferase 1 (Gene Name=Dnmt1)

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