Structure of PDB 6tw1 Chain B

Receptor sequence

>6tw1B (length=740) Species: 1129347 (Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))

MDVNPMLIFLKVPVQNAISTTFPYTGDPPYSHGTGTGYTMDTVIRTHDYS
SRGIWKTNSETGAQQLNPIDGPLPEDNEPSGYAQTDCVLELIEGLDRSHP
GLFETACQETIDAIQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF
RKNGYKLNESGRLIDFLKDVLLSFENDSMEVTTHFQKKKRIRDNHSKKMI
TQRTIGKKRVKLTKKNYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRG
FVYFVELLARNICERLEQSGLPVGGNEKKAKLANVIKKMMAKSTDEELSY
TITGDNTKWNENQNPRIFLAMVLRITAGQPEWFRDLLAVAPIMFSNKVAR
LGRGYMFESKSMHLRTQISAENLSDINLRYFNEDTKKKIEKIRHLMVEGT
ASLSPGMMMGMFNMLSTVLGVSVLNLGQREILKRTYWWDGLQSSDDFALI
INGHFKEDIQQGVNHFYRTCKLVGINMSQKKSYINKTGTFEFTSFFYRYG
FVANFSMELPSFGVAGNNESADMSIGTTVIKTNMINNDLGPATAQMAIQL
FIKDYRYTYRCHRGDTNLETRRTKSIKRLWTETISKAGLLVADGGPNPYN
LRNLHIPEVCLKWSLMDPDYRGRLCNPNNPFVHHMEVSLEYDAVATTHSW
TPKRNRSILNTNQRGILEDERIYQKCCQVFEKFFPSSTYRRPIGMASMLD
AMLSRARIDARIDLESGRISSQDFSEITNTCKAIEALKRQ

3D structure

• PDB: 6tw1 A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	H32 T34 G125 R126 Q127 N136 Q186 D193 S196 K198 I200 R203 K237 I241 A242 T243 R249 R260 P272 G274 R353 M356 R365 G410 N413 S524 T528 K553 Y557 T566 L568 E569 R571 H633 I674 L675 N676	H32 T34 G125 R126 Q127 N136 Q186 D193 S196 K198 I200 R203 K237 I241 A242 T243 R249 R260 P272 G274 R353 M356 R365 G410 N413 S524 T528 K553 Y557 T566 L568 E569 R571 H633 I658 L659 N660
BS02	rna	B	Y24 R126 K229 R239 D305 N310 M409 G410 S444 D445 D446 M507 P510	Y24 R126 K229 R239 D305 N310 M409 G410 S444 D445 D446 M507 P510
BS03	MG	B	D305 D445 D446	D305 D445 D446
BS04	MG	B	D305 N306 D445	D305 N306 D445
BS05	POP	B	K308 W309 K481	K308 W309 K481

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0005737 cytoplasm
• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:6tw1, PDBe:6tw1, PDBj:6tw1
• PDBsum: 6tw1
• PubMed: 32304664
• UniProt: H6QM91