Structure of PDB 6t26 Chain B

Receptor sequence

>6t26B (length=502) Species: 169049 (Vibrio sp. G15-21)

AEIKNVILMIGDGMGPQQVGLLETYANQAPNSIYKGNKTAIYQLAQEGVI
GSSLTHPEDAIVVDSACSATMLATGIYSSSEVIGIDSQGNHVETVLEKAK
KAGKATGLVSDTRLTHATPASFAAHQPHRSLENQIASDMLATGADVMLSG
GLRHWIPKSTNDKGETYKQLEKLTQGDVYLKSKRKDDRNLLTEAEKDGYQ
LAFNRNMLDDAKGDKLLGLFAYSGMDDGIAYSNKKKSGERTQPSLKEMTQ
KALNILSKDEDGFFLMVEGGQIDWAGHSNDAGTMLHELLKFDEAIQTVYE
WAKDREDTIVIVTADHETGSFGFSYSSNDLPKPQKRSGEAFADRDYAPNF
NFGAFDILDGLYNQKQSYYGMISEFQKLDKSLQTPEKLAEIVNKNSEFPI
TAEQAKNVLASKPNPYRLAQHKYLSAEEVPAINDFDAFFPYNDRGNLLAR
EQATGQNIVWGTGTHTHTPVNVFAWGPAEKILPVSKIMHHSELGEYIKQQ
VN

3D structure

• PDB: 6t26 X-ray crystal structure of Vibrio alkaline phosphatase with the non-competitive inhibitor cyclohexylamine.
• Chain: B
• Resolution: 2.265 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D12 S65 H116 T118 R129 E268 D273 W274 H277 D315 H316 H465
• Catalytic site (residue number reindexed from 1): D12 S65 H116 T118 R129 E268 D273 W274 H277 D315 H316 H465

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D273 H277 H465	D273 H277 H465
BS02	ZN	B	D12 S65 D315 H316	D12 S65 D315 H316
BS03	MG	B	D12 T118 E268	D12 T118 E268
BS04	PO4	B	S65 R129 D273 H316 H465	S65 R129 D273 H316 H465
BS05	MG	B	G103 D259 D261	G103 D259 D261
BS06	MG	B	A45 Q46 G48	A45 Q46 G48

Gene Ontology

Molecular Function

• GO:0004035 alkaline phosphatase activity
• GO:0016791 phosphatase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0016311 dephosphorylation

External links

• PDB: RCSB:6t26, PDBe:6t26, PDBj:6t26
• PDBsum: 6t26
• PubMed: 33102813
• UniProt: Q93P54