Structure of PDB 6szv Chain B

Receptor sequence

>6szvB (length=743) Species: 1129347 (Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))

MDVNPMLIFLKVPVQNAISTTFPYTGDPPYSHGTGTGYTMDTVIRTHDYS
SRGIWKTNSETGAQQLNPIDGPLPEDNEPSGYAQTDCVLELIEGLDRSHP
GLFETACQETIDAIQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF
RKNGYKLNESGRLIDFLKDVLLSFENDSMEVTTHFQKKKRIRDNHSKKMI
TQRTIGKKRVKLTKKNYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRG
FVYFVELLARNICERLEQSGLPVGGNEKKAKLANVIKKMMAKSTDEELSY
TITGDNTKWNENQNPRIFLAMVLRITAGQPEWFRDLLAVAPIMFSNKVAR
LGRGYMFESKSMHLRTQISAENLSDINLRYFNEDTKKKIEKIRHLMVEGT
ASLSPGMMMGMFNMLSTVLGVSVLNLGQREILKRTYWWDGLQSSDDFALI
INGHFKEDIQQGVNHFYRTCKLVGINMSQKKSYINKTGTFEFTSFFYRYG
FVANFSMELPSFGVAGNNESADMSIGTTVIKTNMINNDLGPATAQMAIQL
FIKDYRYTYRCHRGDTNLETRRTKSIKRLWTETISKAGLLVADGGPNPYN
LRNLHIPEVCLKWSLMDPDYRGRLCNPNNPFVHHMEVESTSLEYDAVATT
HSWTPKRNRSILNTNQRGILEDERIYQKCCQVFEKFFPSSTYRRPIGMAS
MLDAMLSRARIDARIDLESGRISSQDFSEITNTCKAIEALKRQ

3D structure

• PDB: 6szv A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.
• Chain: B
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	H32 G33 T34 Y38 Q186 K198 I200 R203 K237 M356 R365 S673 I674 L675 N676	H32 G33 T34 Y38 Q186 K198 I200 R203 K237 M356 R365 S660 I661 L662 N663
BS02	rna	B	G125 R126 Q127 R135 N136 M227 I241 A242 T243 R249 R260 P272 R353 G410 M414 I525 T528 H633 T667 K669 R670 S673 R687 Q690 Q694	G125 R126 Q127 R135 N136 M227 I241 A242 T243 R249 R260 P272 R353 G410 M414 I525 T528 H633 T654 K656 R657 S660 R674 Q677 Q681
BS03	rna	B	Y24 N413 S444 D446 M507 P510 R706	Y24 N413 S444 D446 M507 P510 R693
BS04	2KH	B	K229 R239 D305 N306 K308 W309 N310 M409 G410 D445 K481	K229 R239 D305 N306 K308 W309 N310 M409 G410 D445 K481
BS05	MG	B	D305 N306 D445	D305 N306 D445
BS06	MG	B	D305 D445 D446	D305 D445 D446

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0005737 cytoplasm
• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:6szv, PDBe:6szv, PDBj:6szv
• PDBsum: 6szv
• PubMed: 32304664
• UniProt: H6QM91