Structure of PDB 6sls Chain B

Receptor sequence

>6slsB (length=517) Species: 1887 (Streptomyces albogriseolus)

DNRIKTVVILGGGTAGWMTAAYLGKALQNTVKIVVLEAPTIPRIGVGEAT
VPNLQRAFFDYLGIPEEEWMRECNASYKMAVKFINWRTPGEGSPDPRTLD
DGHTDTFHHPFGLLPSADQIPLSHYWAAKRLQGETDENFDEACFADTAIM
NAKKAPRFLDMRRATNYAWHFDASKVAAFLRNFAVTKQAVEHVEDEMTEV
LTDERGFITALRTKSGRILQGDLFVDCSGFRGLLINKAMEEPFIDMSDHL
LCNSAVATAVPHDDEKNGVEPYTSSIAMEAGWTWKIPMLGRFGSGHVYSD
HFATQDEATLAFSKLWGLDPDNTEFNHVRFRVGRNRRAWVRNCVSVGLAS
CFVEPLESSGIYFIYAAIHMLAKHFPDKTFDKVLVDRFNREIEEMFDDTR
DFLQAHYYFSPRVDTPFWRANKELKLADSIKDKVETYRAGLPVNLPVTEF
RNFWTNGSYYCIFAGLGLMPRNPLPALAYKPQSIAEAELLFADVKRKGDT
LVESLPSTYDLLRQLHG

3D structure

• PDB: 6sls Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal is negatively coupled.
• Chain: B
• Resolution: 2.32 Å

Enzymatic activity

• Enzyme Commision number: 1.14.19.59: tryptophan 6-halogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PO4	B	R439 R483	R438 R471
BS02	PO4	B	H250 A428	H249 A427
BS03	FAD	B	G12 G13 G14 T15 A16 A39 I42 R44 I45 V47 G48 E49 A50 E197 M198 C228 S229 L234 W285 L349 F353 P356 G361 I362	G11 G12 G13 T14 A15 A38 I41 R43 I44 V46 G47 E48 A49 E196 M197 C227 S228 L233 W284 L348 F352 P355 G360 I361

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004497 monooxygenase activity
• GO:0016491 oxidoreductase activity

External links

• PDB: RCSB:6sls, PDBe:6sls, PDBj:6sls
• PDBsum: 6sls
• PubMed: 31589794
• UniProt: A1E280|TRP6H_STRAO Tryptophan 6-halogenase ThaL (Gene Name=thaL)