Structure of PDB 6skj Chain B

Receptor sequence

>6skjB (length=390) Species: 9606 (Homo sapiens)

PAKTMEEASKRSYQFWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQ
GFTWDALDLGDRGVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPP
GWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDTEKKMVEINFLCVHKKL
RSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWHRSLNPR
KLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLT
RYLKQFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLP
STIMNHPTHKSLKAAYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALD
LMENKTFLEKLKFGIGDGNLQYYLYNWKCPSMGAEKVGLV

3D structure

• PDB: 6skj High-resolution snapshots of human N-myristoyltransferase in action illuminate a mechanism promoting N-terminal Lys and Gly myristoylation.
• Chain: B
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 2.3.1.-
  2.3.1.97: glycylpeptide N-tetradecanoyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	D183 D185 M187 F188 F190 T282 R295 Y296 H298 F311 S405 I469 G470 D471 G472	D79 D81 M83 F84 F86 T178 R191 Y192 H194 F207 S301 I365 G366 D367 G368
BS02	COA	B	Y117 Q118 F119 W120 N179 Y180 V181 L248 V250 R255 S256 R258 A260 P261 A283 L287	Y13 Q14 F15 W16 N75 Y76 V77 L144 V146 R151 S152 R154 A156 P157 A179 L183
BS03	MYA	B	Y117 Q118 F119 W120 Y180 V181 I245 L248 C249 V250 R255 S256 R258 A260 P261 T268 V271 Y281 T282 L287	Y13 Q14 F15 W16 Y76 V77 I141 L144 C145 V146 R151 S152 R154 A156 P157 T164 V167 Y177 T178 L183

Gene Ontology

Molecular Function

• GO:0004379 glycylpeptide N-tetradecanoyltransferase activity

Biological Process

• GO:0006499 N-terminal protein myristoylation

External links

• PDB: RCSB:6skj, PDBe:6skj, PDBj:6skj
• PDBsum: 6skj
• PubMed: 32111831
• UniProt: P30419|NMT1_HUMAN Glycylpeptide N-tetradecanoyltransferase 1 (Gene Name=NMT1)