Structure of PDB 6rjc Chain B

Receptor sequence

>6rjcB (length=667) Species: 83333 (Escherichia coli K-12)

SSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQN
PSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHP
EVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYA
FMGDGCMMEGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDT
AMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSLLMCKTIIGFGS
PNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQ
AKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMPSDFDAKAKEFIAKLQAN
PAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDAA
GNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALM
KQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAV
AWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPE
LIFIATGSEVELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVL
PKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAPAELLFEEFGFT
VDNVVAKAKELLHHHHH

3D structure

• PDB: 6rjc Low-barrier hydrogen bonds in enzyme cooperativity.
• Chain: B
• Resolution: 1.05 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H26 I247 H261 E411 H473
• Catalytic site (residue number reindexed from 1): H25 I246 H260 E410 H472
• Enzyme Commision number: 2.2.1.1: transketolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	B	D155 N185 I187	D154 N184 I186

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004802 transketolase activity
• GO:0016740 transferase activity
• GO:0030145 manganese ion binding
• GO:0030976 thiamine pyrophosphate binding
• GO:0042803 protein homodimerization activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006098 pentose-phosphate shunt
• GO:0009052 pentose-phosphate shunt, non-oxidative branch

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:6rjc, PDBe:6rjc, PDBj:6rjc
• PDBsum: 6rjc
• PubMed: 31534226
• UniProt: P27302|TKT1_ECOLI Transketolase 1 (Gene Name=tktA)