Structure of PDB 6rie Chain B

Receptor sequence

>6rieB (length=1129) Species: 10245 (Vaccinia virus)

EMDQRLGYKFLVPDPKAGVFYRPLHFQYVSYSNFILHRLHEILTVKRPLL
SFKNNTERIMIEISNVKVTPPDYSPIIASIKGKSYDALATFTVNIFKEVM
TKEGISITKISSYEGLIKIPLLIGYGNKNPLDTAKYLVPNVIGGVFINKQ
SVEKVGINLVEKITTWPKFRVVKPNSFTFSFSSVSPPNVLPTRYRHYKIS
LDISQLEALNISSTKTFITVNIVLLSQYLSRVSLEFIRRSLSYDMPPEVV
YLVNAIIDSAKRITESITDFNIDTYINDLVEAEHIKQKSQLTINEFKYEM
LHNFLPHMNYTPDQLKGFYMISLLRKFLYCIFHTSRYPDRDSMVCHRILT
YGKYFETLAHDELENYIGNIRNDIMNNHKNRGTYAVNIHVLTTPGLNHAF
SSLLSGKFKKSDGSYRTHPHYSWMQNISIPRSVGFYPSVRKYHPSQYLYF
CSSDVPERGPQVGLVSQLSVLSSITNILTSEYLDLEKKICEYIRSYYKDD
ISYFETGFPITIENALVASLNPNMICDFVTDFRRRKRMGFFGNLEVGITL
VRDHMNEIRINIGAGRLVRPFLVVDNGELMMDVCPELESRLDDMTFSDIQ
KEFPHVIEMVDIEQFTFSNVCESVQKFRMMSKDERKQYDLCDFPAEFRDG
YVASSLVGINHNSGPRAILGCAQAKQAISCLSSDIRNKIDNGIHLMYPER
PIVISKALETSKIAANCFGQHVTIALMSYKGINQEDGIIIKKQFIQRGGL
DIVTAKKHQVEIPLENFNNKERDRSNAYSKLESNGLVRLNAFLESGDAMA
RNISSSFDVSEKYTDMYKSRVERVQVELTDKVKVRVLTMKERRPILGDKF
TTRTSQKGTVAYVADETELPYDENGITPDVIINSTSIFSRKTISMLIEVI
LTAAYSAKPYNNKGENRPVCFPSSNETSIDTYMQFAKQCYEHSNPKLSDE
ELSDKIFCEKILYDPETDKPYASKVFFGPIYYLRLRHLTQDKATVRCRGK
KTKLIRQANEGRKRGGGIKFGEMERDCLIAHGAANTITEVLKDSEEDYQD
VYVCENCGDIAAQIKGINTCLRCSKLNLSPLLTKIDTTHVSKVFLTQMNA
RGVKVKLDFERRPPSFYKPLDKVDLKPSF

3D structure

• PDB: 6rie Structural Basis of Poxvirus Transcription: Transcribing and Capping Vaccinia Complexes.
• Chain: B
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	Y432 R478 Q481 Q696 D841 V842 K882 K890 H1020 K1033	Y421 R458 Q461 Q676 D808 V809 K849 K857 H987 K1000
BS02	dna	B	K777 R1045 K1046 K1052 M1056	K757 R1012 K1013 K1019 M1023
BS03	ZN	B	C1087 C1103 C1106	C1054 C1070 C1073

Gene Ontology

Molecular Function

• GO:0001054 RNA polymerase I activity
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006360 transcription by RNA polymerase I

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0044423 virion component

External links

• PDB: RCSB:6rie, PDBe:6rie, PDBj:6rie
• PDBsum: 6rie
• PubMed: 31835031
• UniProt: Q76ZP7|RP132_VACCW DNA-directed RNA polymerase 133 kDa polypeptide (Gene Name=OPG151)