Structure of PDB 6qs6 Chain B

Receptor sequence

>6qs6B (length=582) Species: 562 (Escherichia coli)

QALKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEP
GVGKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEER
LKGVLNDLAKQEGNVILFIDALHTMVGAGKADGAMDAGNMLKPALARGEL
HCVGATTLDEYRQYIEKDAALERRFQKVFVAEPSVEDTIAILRGLKERYE
LHHHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRMQIDSK
LRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEA
VDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSD
EAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILL
DAVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQ
ERFGELDYAHMKELVLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQ
IQLKRLYKRLEERGYEIHISDEALKLLSENGYDPVYGARPLKRAIQQQIE
NPLAQQILSGELVPGKVIRLEVNEDRIVAVQH

3D structure

• PDB: 6qs6 Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor.
• Chain: B
• Resolution: 3.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	K250 Y251 R252 A289 D290 Y653 V654	K92 Y93 R94 A131 D132 Y377 V378
BS02	AGS	B	V180 I181 P208 G211 K212 T213 A214 L353 P387	V22 I23 P50 G53 K54 T55 A56 L195 P229
BS03	AGS	B	R569 V570 I571 G608 V609 G610 K611 T612 E613 N719 I774 A814 R815 K818	R293 V294 I295 G332 V333 G334 K335 T336 E337 N443 I498 A538 R539 K542
BS04	AGS	B	R331 R332	R173 R174

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding

Biological Process

• GO:0009408 response to heat
• GO:0034605 cellular response to heat
• GO:0042026 protein refolding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016020 membrane

External links

• PDB: RCSB:6qs6, PDBe:6qs6, PDBj:6qs6
• PDBsum: 6qs6
• PubMed: 31216466
• UniProt: P63284|CLPB_ECOLI Chaperone protein ClpB (Gene Name=clpB)