Structure of PDB 6qga Chain B

Receptor sequence

>6qgaB (length=558) Species: 1547922 (Piscinibacter sakaiensis)

VPLASRAACEALKDDMVWPNAATVVEVAAWRDAAPATASAAALPEHCEVS
GAIAKRTGIDGYPYEIKFRLRMPAEWNGRFFMEGGSGTNGSLSAATGSIG
GGQIASALSRNFATIATDGGHDNAVNDNPDALGTVAFGLDPQARLDMGYN
SYDQVTQAGKAAVARFYGRAADKSYFIGCSEGGREGMMLSQRFPSHYDGI
VAGAPGYQLPKAGISGAWTTQSLAPAAVGLDAQGVPLINKSFSDADLHLL
SQAILGTCDALDGLADGIVDNYRACQAAFDPATAANPANGQALQCVGAKT
ADCLSPVQVTAIKRAMAGPVNSAGTPLYNRWAWDAGMSGLSGTTYNQGWR
SWWLGSFNSSANNAQRVSGFSARSWLVDFATPPEPMPMTQVAARMMKFDF
DIDPLKIWATSGQFTQSSMDWHGATSTDLAAFRDRGGKMILYHGMSDAAF
SALDTADYYERLGAAMPGAAGFARLFLVPGMNHCSGGPGTDRFDMLTPLV
AWVERGEAPDQISAWSGTPGYFGVAARTRPLCPYPQIARYKGSGDINTEA
NFACAAPP

3D structure

• PDB: 6qga Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate.
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 3.1.1.102: mono(ethylene terephthalate) hydrolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	J1K	B	S131 G132 S225 E226 L254 W397 R411 F415 S416 F495 H528	S86 G87 S180 E181 L209 W352 R366 F370 S371 F450 H483
BS02	CA	B	D304 D307 L309 D311 I313	D259 D262 L264 D266 I268
BS03	J1K	B	A206 A209	A161 A164
BS04	J1K	B	A86 A87 I149	A41 A42 I104

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding
• GO:0052689 carboxylic ester hydrolase activity

Biological Process

• GO:0042178 xenobiotic catabolic process

Cellular Component

• GO:0009279 cell outer membrane

External links

• PDB: RCSB:6qga, PDBe:6qga, PDBj:6qga
• PDBsum: 6qga
• PubMed: 30979881
• UniProt: A0A0K8P8E7|MHETH_PISS1 Mono(2-hydroxyethyl) terephthalate hydrolase (Gene Name=ISF6_0224)