Structure of PDB 6qcw Chain B

Receptor sequence

>6qcwB (length=745) Species: 1601067 (Influenza B virus (B/Memphis/13/2003))

GMNINPYFLFIDVPIQAAISTTFPYTGVPPYSHGTGTGYTIDTVIRTHEY
SNKGKQYISDVTGCTMVDPTNGPLPEDNEPSAYAQLDCVLEALDRMDEEH
PGLFQAASQNAMETLMVTTVDKLTQGRQTFDWTVCRNQPAATALNTTITS
FRLNDLNGADKGGLIPFCQDIIDSLDRPEMTFFSVKNIKKKLPAKNRKGF
LIKRIPMKVKDKITKVEYIKRALSLNTMTKDAERGKLKRRAIATAGIQIR
GFVLVVENLAKNICENLEQSGLPVGGNEKKAKLSNAVAKMLSNCPPGGIS
MTVTGDNTKWNECLNPRIFLAMTERITRDSPIWFRDFCSIAPVLFSNKIA
RLGKGFMITSKTKRLKAQIPCPDLFSIPLERYNEETRAKLKKLKPFFNEE
GTASLSPGMMMGMFNMLSTVLGVAALGIKNIGNKEYLWDGLQSSDDFALF
VNAKDEETCMEGINDFYRTCKLLGINMSKKKSYCNETGMFEFTSMFYRDG
FVSNFAMELPSFGVAGVNESADMAIGMTIIKNNMINNGMGPATAQTAIQL
FIADYRYTYKCHRGDSKVEGKRMKIIKELWENTKGRDGLLVADGGPNIYN
LRNLHIPEIVLKYNLMDPEYKGRLLHPQNPFVGHLSEADITPAHGPVKKM
DYDAVSGTHSWRTKRNRSILNTDQRNMILEEQCYAKCCNLFEACFNSASY
RKPVGQHSMLEAMAHRLRMDARLDYESGRMSKDDFEKAMAHLGEI

3D structure

• PDB: 6qcw Structural snapshots of actively transcribing influenza polymerase.
• Chain: B
• Resolution: 2.88 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	Y24 R126 E277 D444 T492 M506 K652 D655	Y25 R127 E278 D445 T493 M507 K648 D651
BS02	rna	B	G125 R126 Q127 N136 M227 K229 D230 A242 T243 R249 L271 P272 V273 G274 G352 K353 M412 N414 N670 R671 S672 N675	G126 R127 Q128 N137 M228 K230 D231 A243 T244 R250 L272 P273 V274 G275 G353 K354 M413 N415 N666 R667 S668 N671
BS03	rna	B	H32 G33 T34 Y38 K237 M356 K365 E384	H33 G34 T35 Y39 K238 M357 K366 E385

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:6qcw, PDBe:6qcw, PDBj:6qcw
• PDBsum: 6qcw
• PubMed: 31160782
• UniProt: Q5V8Y6