Structure of PDB 6qcv Chain B

Receptor sequence

>6qcvB (length=746) Species: 1601067 (Influenza B virus (B/Memphis/13/2003))

GMNINPYFLFIDVPIQAAISTTFPYTGVPPYSHGTGTGYTIDTVIRTHEY
SNKGKQYISDVTGCTMVDPTNGPLPEDNEPSAYAQLDCVLEALDRMDEEH
PGLFQAASQNAMETLMVTTVDKLTQGRQTFDWTVCRNQPAATALNTTITS
FRLNDLNGADKGGLIPFCQDIIDSLDRPEMTFFSVKNIKKKLPAKNRKGF
LIKRIPMKVKDKITKVEYIKRALSLNTMTKDAERGKLKRRAIATAGIQIR
GFVLVVENLAKNICENLEQSGLPVGGNEKKAKLSNAVAKMLSNCPPGGIS
MTVTGDNTKWNECLNPRIFLAMTERITRDSPIWFRDFCSIAPVLFSNKIA
RLGKGFMITSKTKRLKAQIPCPDLFSIPLERYNEETRAKLKKLKPFFNEE
GTASLSPGMMMGMFNMLSTVLGVAALGIKNIGNKEYLWDGLQSSDDFALF
VNAKDEETCMEGINDFYRTCKLLGINMSKKKSYCNETGMFEFTSMFYRDG
FVSNFAMELPSFGVAGVNESADMAIGMTIIKNNMINNGMGPATAQTAIQL
FIADYRYTYKCHRGDSKVEGKRMKIIKELWENTKGRDGLLVADGGPNIYN
LRNLHIPEIVLKYNLMDPEYKGRLLHPQNPFVGHLSKEADITPAHGPVKK
MDYDAVSGTHSWRTKRNRSILNTDQRNMILEEQCYAKCCNLFEACFNSAS
YRKPVGQHSMLEAMAHRLRMDARLDYESGRMSKDDFEKAMAHLGEI

3D structure

• PDB: 6qcv Structural snapshots of actively transcribing influenza polymerase.
• Chain: B
• Resolution: 3.24 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	Y24 E277 N414 S443 D444 D445 M506 P509 S510 M654 D655	Y25 E278 N415 S444 D445 D446 M507 P510 S511 M651 D652
BS02	rna	B	G125 R126 Q127 N136 M227 K229 I241 A242 T243 R249 L271 P272 V273 G274 G352 M410 G411 N414 M415 N670 R671 S672 N675	G126 R127 Q128 N137 M228 K230 I242 A243 T244 R250 L272 P273 V274 G275 G353 M411 G412 N415 M416 N667 R668 S669 N672
BS03	rna	B	H32 G33 T34 Y38 K237 M356 K365	H33 G34 T35 Y39 K238 M357 K366
BS04	CTP	B	R239 N306 T307 K308 W309 N310 M410 G411 D444	R240 N307 T308 K309 W310 N311 M411 G412 D445
BS05	MG	B	D305 N306	D306 N307

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:6qcv, PDBe:6qcv, PDBj:6qcv
• PDBsum: 6qcv
• PubMed: 31160782
• UniProt: Q5V8Y6