Structure of PDB 6osp Chain B

Receptor sequence
>6ospB (length=310) Species: 9606 (Homo sapiens) [Search protein sequence]
NLYFQSMDPLLSVLMWGVNHSINELSHVQIPVMLMPDDFKAYSKIKVDNH
LFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDRF
HTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLGMYRL
NVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSAREASDKEKAKELPTLKDN
DFINEGQKIYIDDNNKKVFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERA
EQELAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYTVNPEQYSK
RFLDFIGHIL
3D structure
PDB6osp Targeting the PI5P4K Lipid Kinase Family in Cancer Using Covalent Inhibitors.
ChainB
Resolution2.21 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K145 D273
Catalytic site (residue number reindexed from 1) K111 D237
Enzyme Commision number 2.7.1.149: 1-phosphatidylinositol-5-phosphate 4-kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 N51 B K140 I143 K145 N198 V199 F200 L277 K106 I109 K111 N164 V165 F166 L241 MOAD: ic50=190nM
BindingDB: IC50=950nM
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016308 1-phosphatidylinositol-4-phosphate 5-kinase activity
GO:0016309 1-phosphatidylinositol-5-phosphate 4-kinase activity
GO:0042803 protein homodimerization activity
GO:0052742 phosphatidylinositol kinase activity
Biological Process
GO:0006629 lipid metabolic process
GO:0010506 regulation of autophagy
GO:0016310 phosphorylation
GO:0035855 megakaryocyte development
GO:0046488 phosphatidylinositol metabolic process
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0046854 phosphatidylinositol phosphate biosynthetic process
GO:0061909 autophagosome-lysosome fusion
GO:0090119 vesicle-mediated cholesterol transport
GO:1902635 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process
GO:2000786 positive regulation of autophagosome assembly
Cellular Component
GO:0001750 photoreceptor outer segment
GO:0001917 photoreceptor inner segment
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005764 lysosome
GO:0005776 autophagosome
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0042995 cell projection

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6osp, PDBe:6osp, PDBj:6osp
PDBsum6osp
PubMed32130941
UniProtP48426|PI42A_HUMAN Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (Gene Name=PIP4K2A)

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