Structure of PDB 6npl Chain B

Receptor sequence

>6nplB (length=861) Species: 7955 (Danio rerio)

KFGWIKGVLVRCMLNIWGVMLFIRMTWIVGQAGIAYSCIIVIMATVVTTI
TGCSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMY
VVGFAETVVELLMDSGLLMIDQTNDIRVIGTITVILLLGISVAGMEWEAK
AQIFLLVILITAIFNYFIGSFIAVDSKKKFGFFSYDAGILAENFGPDFRG
QTFFSVFSIFFPAATGILAGANISGDLADPQMAIPKGTLLAILITGLVYV
GVAISAGACIVRDATGIESNFTLISNCTDAACKYGYDFSSCRPTVEGEVS
SCKFGLHNDFQVMSVVSGFSPLISAGIFSATLSSALASLVSAPKVFQALC
KDNIYPGIAIFGKGYGKNNEPLRGYFLTFGIALAFILIAELNVIAPIISN
FFLASYALINFSVFHASLANSPGWRPSFKYYNMWASLAGAILCCVVMFII
NWWAALLTNVIVLSLYIYVSYKKPDVNWGSSTQALTYHQALTHSLQLCGV
ADHIKTFRPQCLVMTGAPNSRPAILHLVHAFTKNVGLMLCGHVRISSRRP
NFKELNSDMLRYQRWLLNNNSKAFYTCVVAEDLRQGTQYMLQAAGLGRLR
PNTLVIGFKNDWRIGDIKEVETYINLIHDAFDFQYGVVILRLREGLDISH
LNVADQRLLDSQQFQQKQGKGTVDVWWLFDDGGLTLLIPYLIANKKKWKD
CKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDITVLGDINTKPKSEGL
TEFAEMIEPYKLREDDMEQEAAEKLKSEEPWRITDNELELYKAKGNRQIR
LNELLKEHSSTANLIVMSMPLARKGAVSSALYMAWLDTLSKDLPPILLVR
GNHQSVLTFYS

3D structure

• PDB: 6npl Structure and mechanism of the cation-chloride cotransporter NKCC1.
• Chain: B
• Resolution: 2.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	K	B	N220 I221 Y305 P417 A418 T420 G421	N15 I16 Y100 P212 A213 T215 G216
BS02	CL	B	G421 L423	G216 L218

Gene Ontology

Molecular Function

• GO:0015377 chloride:monoatomic cation symporter activity
• GO:0022857 transmembrane transporter activity

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0055085 transmembrane transport

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:6npl, PDBe:6npl, PDBj:6npl
• PDBsum: 6npl
• PubMed: 31367042
• UniProt: A0A0G2KTI4|S12A2_DANRE Solute carrier family 12 member 2 (Gene Name=slc12a2)