Structure of PDB 6lq6 Chain B

Receptor sequence

>6lq6B (length=609) Species: 1772 (Mycolicibacterium smegmatis)

SMSHYKSNVRDQVFNLFEVFGVDKVLGADKFSDLDADTAREMLTEIARLA
EGPIAESFVEGDRNPPVFDPETHTVTLPEGFKKSMRALFDGGWDKVGLAE
HLGGIPMPRALQWALIEHILGANPAAYMYAMGPGMSEIFYNNGTDEQKKW
ATIAAERGWGATMVLTEPDAGSDVGAGRTKAVQQPDGTWHIEGVKRFITS
ADSDDLFENIMHLVLARPEGAGPGTKGLSLFFVPKFHFDHETGEIGERNG
VFVTNVEHKMGLKVSATCELSLGQHGIPAVGWLVGEVHNGIAQMFDVIEQ
ARMMVGTKAIATLSTGYLNALEYAKERVQGADMTQMTDKTAPRVTITHHP
DVRRSLMTQKAYAEGLRAIYLYTATFQDAEVAQAVHGVDGDLAARVNDLL
LPIVKGFGSETAYAKLTESLQTLGGSGFLQDYPIEQYIRDSKIDSLYAGT
TAIQAQDFFFRKIIRDKGQALAYVAGEIEQFIKNNGRLKTERELLATALA
DVQGMAASLTGYLMAAQEDAASIYKVGLGSVRFLMAVGDLLSGWLLARQA
AVAIEKLDAGATGADKSFYEGKIAAASFFAKNMLPLLTSTRQIIENLDND
VMELDEAAF

3D structure

• PDB: 6lq6 Structural basis for the broad substrate specificity of two acyl-CoA dehydrogenases FadE5 from mycobacteria.
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 1.3.8.1: short-chain acyl-CoA dehydrogenase.
  1.3.8.7: medium-chain acyl-CoA dehydrogenase.
  1.3.8.8: long-chain-acyl-CoA dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	R326 I345 H348 Q420 T421 G423 G424	R327 I346 H349 Q421 T422 G424 G425
BS02	FAD	B	M162 L164 T165 G170 S171 F196 T198 I442 Y446 T449	M163 L165 T166 G171 S172 F197 T199 I443 Y447 T450
BS03	DCR	B	Q111 M130 G133 M134 A160 T161 T198 V296 Q299 Y446 A447	Q112 M131 G134 M135 A161 T162 T199 V297 Q300 Y447 A448
BS04	COA	B	M162 L164 S171 V173 T224 K225 I290 F294 R301 A447 G448 I452 D456 R460 K461	M163 L165 S172 V174 T225 K226 I291 F295 R302 A448 G449 I453 D457 R461 K462

Gene Ontology

Molecular Function

• GO:0004466 long-chain fatty acyl-CoA dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
• GO:0016937 short-chain fatty acyl-CoA dehydrogenase activity
• GO:0070991 medium-chain fatty acyl-CoA dehydrogenase activity

Biological Process

• GO:0006631 fatty acid metabolic process

Cellular Component

• GO:0005886 plasma membrane

External links

• PDB: RCSB:6lq6, PDBe:6lq6, PDBj:6lq6
• PDBsum: 6lq6
• PubMed: 32601219
• UniProt: Q3L887|FADE5_MYCS2 Broad-specificity linear acyl-CoA dehydrogenase FadE5 (Gene Name=fadE5)