Structure of PDB 6k5z Chain B

Receptor sequence

>6k5zB (length=314) Species: 178306 (Pyrobaculum aerophilum str. IM2)

MEIRKDPFTGEYILVSPHRLKRPWQPEGACPFCPGAPETGRGWDVLILPN
RYPVVTENPPEPTAEDLYEVIPARGSSLVVVETPQHDVDDLSDLPLGQIK
KILTAVAEAQRKAEKEGNAAYFLFFRNKGKEIGVSLTHPHSQIYILPVVP
PRVRAELQASYEWYVKHGSCLHCRIVEKEEKRLVFQNRNWKAFVPFYAKW
PHEVHIYPKRHRSLLTELTDEEVADLAEALKITLCALKQVAGIPMPYIMV
LHQAPLPRPTQYYHLHFEIYGMYRPDGKLKHAAGAELGASLFTLDTTPEE
TAARIKAALQKCLK

3D structure

• PDB: 6k5z Unique active site formation in a novel galactose 1-phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum.
• Chain: B
• Resolution: 2.33 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C30 C33 H86 N127 S135 H138 H140 Q142
• Catalytic site (residue number reindexed from 1): C30 C33 H86 N127 S135 H138 H140 Q142

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	C30 C33 H86 H138	C30 C33 H86 H138
BS02	ZN	B	C170 C173 H211 H264	C170 C173 H211 H264
BS03	FE	B	E156 H252 H266 E268	E156 H252 H266 E268
BS04	PO4	B	V134 S135 L136 H140	V134 S135 L136 H140

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008108 UDP-glucose:hexose-1-phosphate uridylyltransferase activity
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0006012 galactose metabolic process
• GO:0033499 galactose catabolic process via UDP-galactose

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:6k5z, PDBe:6k5z, PDBj:6k5z
• PDBsum: 6k5z
• PubMed: 31693208
• UniProt: Q8ZXN7