Structure of PDB 6k4p Chain B

Receptor sequence
>6k4pB (length=1323) Species: 301447 (Streptococcus pyogenes serotype M1) [Search protein sequence]
KKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRILYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDTKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILL
SDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQQLPEKYKEIFF
DQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQ
RTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYV
GPLARGNSRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNL
PNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLL
FKTNRKVTVKQLKEDYFKKIEEFDSVEISGVEDRFNASLGTYHDLLKIIK
DKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLK
RRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQLIHDDSL
TFKEDIQKAQVSSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKP
ENIVIEMARQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLY
YLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNR
GKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVS
DFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGGKA
TAKYFFYSNIMNFFKTEIKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVA
YSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEV
KKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNELALPSKYVNFLYLAS
HYEKPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAY
NKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGG
3D structure
PDB6k4p Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease.
ChainB
Resolution2.9 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna B K33 L35 G56 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 N77 Y81 L101 S104 F105 K111 R115 H116 I122 V126 H129 P133 T134 I135 H160 K163 F164 R165 G166 F168 Y325 H328 H329 K336 R340 F351 F352 Y359 A360 I363 K401 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 R467 T472 I473 P475 K510 Y515 N588 R661 H721 K735 Q739 K742 K929 K1097 E1099 V1100 T1102 G1103 G1104 F1105 I1110 R1122 K1123 K1124 K1130 Y1131 M1169 H1349 Q1350 S1351 Y1356 T1358 K31 L33 G54 E55 T56 A57 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 N75 Y79 L99 S102 F103 K109 R113 H114 I120 V124 H127 P131 T132 I133 H158 K161 F162 R163 G164 F166 Y323 H326 H327 K334 R338 F349 F350 Y357 A358 I361 K399 Q400 R401 T402 F403 N405 G406 F444 R445 I446 Y448 G451 P452 L453 A454 R455 N457 S458 F460 W462 R465 T470 I471 P473 K508 Y513 N586 R659 H715 K729 Q733 K736 K915 K1057 E1059 V1060 T1062 G1063 G1064 F1065 I1070 R1082 K1083 K1084 K1090 Y1091 M1129 H1305 Q1306 S1307 Y1312 T1314
BS02 dna B Y450 F491 M495 T496 N497 K526 G582 E584 E627 W659 R661 N692 I694 Q695 A728 K902 R925 Q926 K1107 E1108 S1109 K1334 Y448 F489 M493 T494 N495 K524 G580 E582 E625 W657 R659 N690 I692 Q693 A722 K888 R911 Q912 K1067 E1068 S1069 K1290
BS03 dna B Q774 K1107 R1114 K1118 D1135 S1136 A1215 S1216 A1320 R1333 R1335 Q760 K1067 R1074 K1078 D1095 S1096 A1175 S1176 A1276 R1289 R1291
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6k4p, PDBe:6k4p, PDBj:6k4p
PDBsum6k4p
PubMed31603896
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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