Structure of PDB 6jki Chain B

Receptor sequence
>6jkiB (length=244) Species: 208963 (Pseudomonas aeruginosa UCBPP-PA14) [Search protein sequence]
SMLWVGVVSIFPEMFRAISDYGITSRAVKQGLLTLTCWNPRVYTEDRHQT
VDDRPFGGGPGMVMKIKPLEGALADARQAAGGRKAKVIYLSPQGRQLTQA
GVRELAEEEALILIAGRYEGIDERFIEEHVDEEWSIGDYVLSGGELPAMV
LVDAVTRLLPGALSAEEDSFTDGLLDCPHYTRPEVYADKRVPEVLLSGNH
EHIRRWRLQQALGRTWERRADLLDSRSLSGEEQKLLAEYIRQRD
3D structure
PDB6jki Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD fromPseudomonas aeruginosa.
ChainB
Resolution2.59 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) P94 E121 R159 D174
Catalytic site (residue number reindexed from 1) P92 E119 R157 D168
Enzyme Commision number 2.1.1.228: tRNA (guanine(37)-N(1))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SFG B D174 S175 D182 D168 S169 D176 PDBbind-CN: -logKd/Ki=6.39,Kd=0.41uM
BS02 SFG B L92 S93 P94 G118 Y120 E121 I138 L143 G145 G146 P149 L90 S91 P92 G116 Y118 E119 I136 L141 G143 G144 P147 PDBbind-CN: -logKd/Ki=6.39,Kd=0.41uM
BS03 MN B E173 D182 H185 E167 D176 H179
Gene Ontology
Molecular Function
GO:0008168 methyltransferase activity
GO:0052906 tRNA (guanine(37)-N1)-methyltransferase activity
Biological Process
GO:0002939 tRNA N1-guanine methylation
GO:0006400 tRNA modification
GO:0008033 tRNA processing
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:6jki, PDBe:6jki, PDBj:6jki
PDBsum6jki
PubMed31399541
UniProtQ9HXQ1|TRMD_PSEAE tRNA (guanine-N(1)-)-methyltransferase (Gene Name=trmD)

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