Structure of PDB 6i01 Chain B

Receptor sequence

>6i01B (length=516) Species: 9606 (Homo sapiens)

LKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDR
FEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLST
QWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDKNKPNDWT
VPKGCFMANVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKFL
TNGSVSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTVT
RDLVTDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAH
MAAFFAASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQAI
STLVRAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVFMNKHDWYEEYP
TTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPL
YDTGSGTIYDLRHFMLGIAPNLARWDFHTTHINQLQLLSTIDESPVFKEF
VKRWKSYLKGSRAKHN

3D structure

• PDB: 6i01 Substrate binding mode and catalytic mechanism of human heparan sulfate d-glucuronyl C5 epimerase.
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 5.1.3.17: heparosan-N-sulfate-glucuronate 5-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GNS	B	Q202 R428 Y500 Y560 R563 R575 F578	Q101 R327 Y399 Y459 R462 R474 F477
BS02	BDP	B	Q202 M445 E499 Y500 N510 Y560 F578	Q101 M344 E398 Y399 N409 Y459 F477
BS03	GNS	B	Y180 R185 R187 Y210 Q213 Q216 M445 Y514	Y79 R84 R86 Y109 Q112 Q115 M344 Y413
BS04	BDP	B	Y180 R185	Y79 R84
BS05	MAN	B	I233 N393	I132 N292
BS06	CA	B	T238 E240 T268 N269 D392	T137 E139 T167 N168 D291

Gene Ontology

Molecular Function

• GO:0047464 heparosan-N-sulfate-glucuronate 5-epimerase activity

Biological Process

• GO:0015012 heparan sulfate proteoglycan biosynthetic process

External links

• PDB: RCSB:6i01, PDBe:6i01, PDBj:6i01
• PDBsum: 6i01
• PubMed: 30872481
• UniProt: O94923|GLCE_HUMAN D-glucuronyl C5-epimerase (Gene Name=GLCE)