Structure of PDB 6hlr Chain B

Receptor sequence
>6hlrB (length=1164) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
TADFRTLERESRFINPPKDKSAFPLLQEAVQPHIGSFNALTEGPDGGLLN
LGVKDIGEKVIFDGKPGYLGNKLSVSVEQVSIAKPMSNDAVERKVYPSES
RQRLTSYRGKLLLKLKWSVNNGEENLFEVRDCGGLPVMLQSNRCHLNKMS
PYELVQHKEESDEIGGYFIVNGIEKLIRMLIVQRRNHPMAIIRPSFANRG
ASYSHYGIQIRSVRPDQTSQTNVLHYLNDGQVTFRFSWRKNEYLVPVVMI
LKALCHTSDREIFDGIIGNDVKDSFLTDRLELLLRGFKKRYPHLQNRTQV
LQYLGDKFRVVFQASPDQSDLEVGQEVLDRIVLVHLGKDGSQDKFRMLLF
MIRKLYSLVAGECSPDNPDATQHQEVLLGGFLYGMILKEKIDEYLQNIIA
QVRMDINRGMAINFKDKRYMSRVLMRVNENIGSKMQYFLSTGNLVSQSGL
DLQQVSGYTVVAEKINFYRFISHFRMVHRGSFFAQLKTTTVRKLLPESWG
FLCPVHTPDGSPCGLLNHFAHKCRISTQQSDVSRIPSILYSLGVAPASHT
FAAGPSLCCVQIDGKIIGWVSHEQGKIIADTLRYWKVEGKTPGLPIDLEI
GYVPPSTRGQYPGLYLFGGHSRMLRPVRYLPLDKEDIVGPFEQVYMNIAV
TPQEIQNNVHTHVEFTPTNILSILANLTPFSDFNQSPRNMYQCQMGKQTM
GTPGVALCHRSDNKLYRLQTGQTPIVKANLYDDYGMDNFPNGFNAVVAVI
SYTGYDMDDAMIINKSADERGFGYGTMYKTEKVDLALNRNRGDPITQHFG
FGNDEWPKEWLEKLDEDGLPYIGTYVEEGDPICAYFDDTLNKTKIKTYHS
SEPAYIEEVNLIGDESNKFQELQTVSIKYRIRRTPQIGDKFSSRHGQKGV
CSRKWPTIDMPFSETGIQPDIIINPHAFPSRMTIGMFVESLAGKAGALHG
IAQDSTPWIFNEDDTPADYFGEQLAKAGYNYHGNEPMYSGATGEELRADI
YVGVVYYQRLRHMVNDKFQVRSTGPVNSLTMQPVKGRKRHGGIRVGEMER
DALIGHGTSFLLQDRLLNSSDYTQASVCRECGSILTTQQSVPRIGSISTV
CCRRCSMRFEDAKIDDSQIWEDGQGNKFVGGNETTTVAIPFVLKYLDSEL
SAMGIRLRYNVEPK
3D structure
PDB6hlr The cryo-EM structure of a 12-subunit variant of RNA polymerase I reveals dissociation of the A49-A34.5 heterodimer and rearrangement of subunit A12.2.
ChainB
Resolution3.18 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna B R495 Q724 K916 K924 H1038 R1065 R469 Q698 K890 K898 H1012 R1039
BS02 dna B S221 Q479 F508 R817 S195 Q453 F482 R791
BS03 dna B N197 I199 M430 N454 Y463 N739 Q1045 R1063 R1070 N171 I173 M404 N428 Y437 N713 Q1019 R1037 R1044
BS04 ZN B C1128 C1131 C1102 C1105
BS05 G2P B R714 R957 R688 R931
Gene Ontology
Molecular Function
GO:0001054 RNA polymerase I activity
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006360 transcription by RNA polymerase I
GO:0006361 transcription initiation at RNA polymerase I promoter
GO:0006362 transcription elongation by RNA polymerase I
GO:0006363 termination of RNA polymerase I transcription
GO:0042254 ribosome biogenesis
GO:0042790 nucleolar large rRNA transcription by RNA polymerase I
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005736 RNA polymerase I complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6hlr, PDBe:6hlr, PDBj:6hlr
PDBsum6hlr
PubMed30913026
UniProtP22138|RPA2_YEAST DNA-directed RNA polymerase I subunit RPA135 (Gene Name=RPA135)

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