Structure of PDB 6gau Chain B

Receptor sequence

>6gauB (length=1068) Species: 83332 (Mycobacterium tuberculosis H37Rv)

IGSTGERGLHHLIWEVVDNAVDEAMAGYATTVNVVLLEDGGVEVADDGRG
IPVATHASGIPTVDVVMTQLGVSVVNALSTRLEVEIKRDGYEWSQVYEKS
EPLGLKQGAPTKKTGSTVRFWADPAVFETTEYDFETVARRLQEMAFLNKG
LTINLTDERVTQDEVVDEVVSDVAEAPVKSRTFHYPGGLVDFVKHINRTK
NAIHSSIVDFSGKGTGHEVEIAMQWNAGYSESVHTFANTINTHEGGTHEE
GFRSALTSVVNKYAKDRGDDIREGLAAVISVKVSEPQFEGQTKTKLGNTE
VKSFVQKVCNEQLTHWFEANPTDAKVVVNKAVSSAQARIAARKSATDIGG
LPGKLADCRSTDPRKSELYVVEGDSAGGSAKSGRDSMFQAILPLRGKIIN
VEKARIDRVLKNTEVQAIITALGTGIHDEFDIGKLRYHKIVLMADADVDG
QHISTLLLTLLFRFMRPLIENGHVFLAQPPLYKLKWQRSDPEFAYSDRER
DGLLEAGLKAGKKINKEDGIQRYKGLGEMDAKELWETTMDPSVRVLRQVT
LDDAAAADELFSILMGEDVDARRSFITRNAKDIEPVDIEQEMQRSYIDYA
MSVIVGRALPEVRDGLKPVHRRVLYAMFDSGFRPDRSHAKSARSVAETMG
NYHPHGDASIYDSLVRMAQPWSLRYPLVDGQGNFGSPGNDPPAAMRYTEA
RLTPLAMEMLREIDEETVDFIPNYDGRVQEPTVLPSRFPNLLANGSGGIA
VGMATNIPPHNLRELADAVFWALENHDADEEETLAAVMGRVKGPDFPTAG
LIVGSQGTADAYKTGRGSIRMRGVVEVEEDSRGRTSLVITELPYQVNHDN
FITSIAEQVRDGKLAGISNIEDQSSDRVGLRIVIEIKRDAVAKVVINNLY
KHTQLQTSFGANMLAIVDGVPRTLRLDQLIRYYVDHQLDVIVRRTTYRLR
KANERAHILRGLVKALDALDEVIALIRASETVDIARAGLIELLDIDEIQA
QAILDMQLRRLAALERQRIIDDLAKIEAEIADLEDILAKPERQRGIVRDE
LAEIVDRHGDDRRTRIIA

3D structure

• PDB: 6gau Overall Structures of Mycobacterium tuberculosis DNA Gyrase Reveal the Role of a Corynebacteriales GyrB-Specific Insert in ATPase Activity.
• Chain: B
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	B	N52 G83 I84 V99 G124 V125	N19 G50 I51 V66 G71 V72
BS02	MG	B	E48 N52	E15 N19

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003916 DNA topoisomerase activity
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0034335 DNA negative supercoiling activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006261 DNA-templated DNA replication
• GO:0006265 DNA topological change
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005694 chromosome
• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:6gau, PDBe:6gau, PDBj:6gau
• PDBsum: 6gau
• PubMed: 30744994
• UniProt: P9WG45|GYRB_MYCTU DNA gyrase subunit B (Gene Name=gyrB);
  P9WG47|GYRA_MYCTU DNA gyrase subunit A (Gene Name=gyrA)