Structure of PDB 6fws Chain B

Receptor sequence

>6fwsB (length=683) Species: 562 (Escherichia coli)

ALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAGEEGRHLA
IEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLK
KIIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQE
EQKRCAKLKGDLDTYKWDGLRDHTDIAIDDDLWRRLSTCPFFVARREIQE
AEVVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAE
ITAPWYRLQLDLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIA
SLNNILNLYMPAGQEAEHRFAMGELPDEVLEICQRLAKLTEMLRGLAELF
LNDLSEKDIVRLHRLILQMNRALGMFEAQSKLWRLASLAQSSGAPVTKWA
TREEREGQLHLWFHCVGIRVSDQLERLLWRSIPHIIVTSATLRSLNSFSR
LQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVEPSIDNEEQHIAE
MAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGDQPRY
RLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDS
PVVITEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIY
DKRLLTKNYGKRLLDALPVFPIEQPEVPEGIVK

3D structure

• PDB: 6fws DNA translocation mechanism of an XPD family helicase.
• Chain: B
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	N85 V86 F113 G114 R117 N223 A225 S543 R545 Q564 R569 S593 K612 F615 P617 I618 Y636 F638 E639 N678 Y679	N84 V85 F112 G113 R116 N207 A209 S523 R525 Q544 R549 S573 K592 F595 P597 I598 Y616 F618 E619 N658 Y659
BS02	SF4	B	R115 C120 P121 C205 F208	R114 C119 P120 C189 F192
BS03	ADP	B	F25 I26 R28 Q31 G57 G59 K60 T61 K95 D599 R659	F24 I25 R27 Q30 G56 G58 K59 T60 K94 D579 R639
BS04	BEF	B	T56 K60 R659	T55 K59 R639

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
• GO:0016853 isomerase activity
• GO:0016887 ATP hydrolysis activity
• GO:0033677 DNA/RNA helicase activity
• GO:0043139 5'-3' DNA helicase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006281 DNA repair
• GO:0009432 SOS response
• GO:0032508 DNA duplex unwinding
• GO:0044806 G-quadruplex DNA unwinding

External links

• PDB: RCSB:6fws, PDBe:6fws, PDBj:6fws
• PDBsum: 6fws
• PubMed: 30520735
• UniProt: P27296|DING_ECOLI ATP-dependent DNA helicase DinG (Gene Name=dinG)