Structure of PDB 6fb0 Chain B

Receptor sequence
>6fb0B (length=154) Species: 3055 (Chlamydomonas reinhardtii) [Search protein sequence]
NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSGKFKHKLSLTFKVTQKTQR
RWFLDKLVDEIGVGYVYDSGSVSNYYLSEIKPLHNFLTQLQPFLKLKQKQ
ANLVLKIIEQLPSAKESPDKFLEVCTWVDQVAALNDSKTRKTTSETVRAV
LDSL
3D structure
PDB6fb0 Understanding the indirect DNA read-out specificity of I-CreI Meganuclease.
ChainB
Resolution2.15 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G19 D20
Catalytic site (residue number reindexed from 1) G18 D19
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B K38 Y66 Y68 I81 K116 D137 K139 K37 Y65 Y67 I80 K115 D136 K138 PDBbind-CN: Kd=100nM
BS02 dna B D20 G21 S22 I24 Q26 K28 T46 Q47 K48 R51 V73 N136 D137 S138 T140 R141 K142 D19 G20 S21 I23 Q25 K27 T45 Q46 K47 R50 V72 N135 D136 S137 T139 R140 K141 PDBbind-CN: Kd=100nM
BS03 CA B A134 N136 A133 N135
Gene Ontology
Molecular Function
GO:0004519 endonuclease activity
GO:0042802 identical protein binding
GO:0046872 metal ion binding
Biological Process
GO:0006314 intron homing
Cellular Component
GO:0009507 chloroplast

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Biological Process
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Cellular Component
External links
PDB RCSB:6fb0, PDBe:6fb0, PDBj:6fb0
PDBsum6fb0
PubMed29980759
UniProtP05725|DNE1_CHLRE DNA endonuclease I-CreI

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