Structure of PDB 6eck Chain B

Receptor sequence
>6eckB (length=532) Species: 10116 (Rattus norvegicus) [Search protein sequence]
AQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATI
GPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATS
PLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTR
GDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGG
ILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDV
LAVRDALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEI
PAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANA
VLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRA
APLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVI
AVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRG
FLRVGDLVIVVTGWRPGSGYTNIMRVLSVSHH
3D structure
PDB6eck Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production.
ChainB
Resolution2.36 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R85 R132 K282 T340
Catalytic site (residue number reindexed from 1) R72 R119 K269 T327
Enzyme Commision number 2.7.1.40: pyruvate kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FBP B T444 K445 T446 S449 W494 R501 G526 R528 G530 S531 G532 Y533 T534 T431 K432 T433 S436 W481 R488 G513 R515 G517 S518 G519 Y520 T521
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003824 catalytic activity
GO:0004743 pyruvate kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0030955 potassium ion binding
GO:0046872 metal ion binding
GO:0048029 monosaccharide binding
Biological Process
GO:0001666 response to hypoxia
GO:0006096 glycolytic process
GO:0007584 response to nutrient
GO:0009749 response to glucose
GO:0010038 response to metal ion
GO:0016310 phosphorylation
GO:0032869 cellular response to insulin stimulus
GO:0033198 response to ATP
GO:0042866 pyruvate biosynthetic process
GO:0051591 response to cAMP
GO:0051707 response to other organism
GO:0071872 cellular response to epinephrine stimulus
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6eck, PDBe:6eck, PDBj:6eck
PDBsum6eck
PubMed31825824
UniProtP12928|KPYR_RAT Pyruvate kinase PKLR (Gene Name=Pklr)

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