Structure of PDB 6e8o Chain B

Receptor sequence

>6e8oB (length=534) Species: 269800 (Thermobifida fusca YX)

GCTPWPAEFAVRYREAGYWTGETFSDFVTDRTRRFADRLAVVGAGQRWTY
AELGERSAVLATGLARLGIAAGDRVVVQLPNIPELFEVVFALFRLGALPV
YALPAHRAHEITHLCTTAQAKALIIPDRHAGFDYRTMAAQLRHAGTAPEH
VVVVGEPGGFTPLAELRADRPDPGVFTRPEASDAAFLQLSGGTTGLPKLI
PRTHDDYLYSVRASAEICALGTDTVYLAALPAVHNFPMSSPGFLGTFHAG
GTVVLAPNPSPDTAFSLIETERVTITAVVPPIALQWLDAVEHGSQSHRDL
SSLRVLQVGGAKFAPEAARRVRPVLGCTLQQVFGMAEGLVNYTRLDDPDD
IITTTQGRPISPDDEIRIVDEADRPVPDGEVGHLLTRGPYTIRGYYRAEE
HNATAFTPDGFYRTGDLVRRTPTGHLVVEGRAKDQINRGGEKVSAEEVEN
HILAHPAVHDAAVVGMSDPYLGERVCAYVIAPPSRSELLRFLRERGLASY
KIPDRVEFVDRFPVTGVGKISRSELRRELARRLD

3D structure

• PDB: 6e8o Implication of MbtH-like proteins in crystallization of the independent NRPS A domains. Crystal structure of FscC: supporting rationale for revised mechanism of freestanding aryl acid adenylating enzymes
• Chain: B
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S193 S213 H237 A339 E340 N440 K445 K525
• Catalytic site (residue number reindexed from 1): S190 S210 H234 A336 E337 N437 K442 K519

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AMP	B	H237 G313 A314 K315 V335 F336 G337 M338 A339 D419 V431 K445	H234 G310 A311 K312 V332 F333 G334 M335 A336 D416 V428 K442

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008668 2,3-dihydroxybenzoate--[aryl-carrier protein] ligase
• GO:0016874 ligase activity

Biological Process

• GO:0019290 siderophore biosynthetic process

External links

• PDB: RCSB:6e8o, PDBe:6e8o, PDBj:6e8o
• PDBsum: 6e8o
• UniProt: Q47NR5