Structure of PDB 6e1j Chain B

Receptor sequence

>6e1jB (length=408) Species: 3707 (Brassica juncea)

ERWPEYIPNKLPDKNYVRVFDTTLRDGEQSPGAALTPPQKIEIARQLAKL
RVDIMEVGFPVSSEEEFETIQTIAKTVGNEVDEETGYIPVICVIARSKER
DIKAAWESVKYAKRPRIVIFTSTSDIHLKYKLKMTREEVVDMVASSIRFA
KSLGFEDIEFGCEDGGRSDKDYICTVFEEAIKAGATTLACPDTVGINMPH
EYGKLVRYIKANTPGIDDVIFSAHCHNDLGVATANTIAGICAGARQVEVT
INGIGERSGNAPLEEVVMALKCRGAFVMGGVYTRIDTRQIMATSKMVQEY
TGLYVQPHKPIVGANCFVHESGIHQDGILKNRSTYEIISPEDVGVVKSQN
SGIVLGKLSGRHAVKGRLKELGYEISDEKLNEVFSRFRDLTKQKKRVTDD
DLKALVTC

3D structure

• PDB: 6e1j Molecular Basis of the Evolution of Methylthioalkylmalate Synthase and the Diversity of Methionine-Derived Glucosinolates.
• Chain: B
• Resolution: 2.096 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	COA	B	Q93 F123 S126 R160 F184 K195	Q29 F59 S62 R96 F120 K131
BS02	MN	B	D90 H288 H290	D26 H224 H226
BS03	KMT	B	R89 P255 T257 H288 H290	R25 P191 T193 H224 H226
BS04	COA	B	S385 G386 I387 D390 K394 L422 R425 H426	S321 G322 I323 D326 K330 L358 R361 H362

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003852 2-isopropylmalate synthase activity
• GO:0016740 transferase activity
• GO:0046872 metal ion binding
• GO:0046912 acyltransferase activity, acyl groups converted into alkyl on transfer

Biological Process

• GO:0009098 L-leucine biosynthetic process
• GO:0019752 carboxylic acid metabolic process
• GO:0043436 oxoacid metabolic process

Cellular Component

• GO:0009507 chloroplast

External links

• PDB: RCSB:6e1j, PDBe:6e1j, PDBj:6e1j
• PDBsum: 6e1j
• PubMed: 31023839
• UniProt: C5J4P1