Structure of PDB 6cst Chain B

Receptor sequence

>6cstB (length=442) Species: 9606 (Homo sapiens)

GLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQ
QKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRD
NPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPP
NFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKR
RYFIKNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDK
NKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTEL
YQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEIN
KAEEQYSLCQELCSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSS
VVSTAEEIFAIAKELLKTEIDADFPHPLRLRLMGVRISSFPN

3D structure

• PDB: 6cst 2.0 angstrom resolution crystal structure of human pol kappa reveals a new catalytic function of N-clasp in DNA replication.
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	I165 I166	I146 I147
BS02	dna	B	S196 K321 G356 G358 V360 T361 R454 T469 R470 A471 S472 T473	S177 K245 G280 G282 V284 T285 R378 T393 R394 A395 S396 T397
BS03	dna	B	T44 F49 M133 S134 M135 A151 P153 F155 I156 S388 S391 R413 K414 S415 M416 S417 V418 E419 R420 T421 R507	T25 F30 M114 S115 M116 A132 P134 F136 I137 S312 S315 R337 K338 S339 M340 S341 V342 E343 R344 T345 R431
BS04	MG	B	D107 M108 D198	D88 M89 D179
BS05	MG	B	D107 D198 E199	D88 D179 E180
BS06	DZ4	B	D107 M108 D109 A110 F111 Y112 S137 T138 Y141 R144 D198 K328	D88 M89 D90 A91 F92 Y93 S118 T119 Y122 R125 D179 K252

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:6cst, PDBe:6cst, PDBj:6cst
• PDBsum: 6cst
• PubMed: 30310122
• UniProt: Q9UBT6|POLK_HUMAN DNA polymerase kappa (Gene Name=POLK)